Volumetric analysis and morphological assessment of the ascending olfactory pathway in an elasmobranch and a teleost usi
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ORIGINAL ARTICLE
Volumetric analysis and morphological assessment of the ascending olfactory pathway in an elasmobranch and a teleost using diceCT Victoria Camilieri‑Asch1,2,8 · Jeremy A. Shaw3 · Kara E. Yopak4 · Lucille Chapuis5 · Julian C. Partridge2 · Shaun P. Collin2,6,7 Received: 28 February 2020 / Accepted: 31 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The size (volume or mass) of the olfactory bulbs in relation to the whole brain has been used as a neuroanatomical proxy for olfactory capability in a range of vertebrates, including fishes. Here, we use diffusible iodine-based contrast-enhanced computed tomography (diceCT) to test the value of this novel bioimaging technique for generating accurate measurements of the relative volume of the main olfactory brain areas (olfactory bulbs, peduncles, and telencephalon) and to describe the morphological organisation of the ascending olfactory pathway in model fish species from two taxa, the brownbanded bamboo shark Chiloscyllium punctatum and the common goldfish Carassius auratus. We also describe the arrangement of primary projections to the olfactory bulb and secondary projections to the telencephalon in both species. Our results identified substantially larger olfactory bulbs and telencephalon in C. punctatum compared to C. auratus (comprising approximately 5.2% vs. 1.8%, and 51.8% vs. 11.8% of the total brain volume, respectively), reflecting differences between taxa, but also possibly in the role of olfaction in the sensory ecology of these species. We identified segregated primary projections to the bulbs, associated with a compartmentalised olfactory bulb in C. punctatum, which supports previous findings in elasmobranch fishes. DiceCT imaging has been crucial for visualising differences in the morphological organisation of the olfactory system of both model species. We consider comparative neuroanatomical studies between representative species of both elasmobranch and teleost fish groups are fundamental to further our understanding of the evolution of the olfactory system in early vertebrates and the neural basis of olfactory abilities. Keywords Brain · Neuroanatomy · Morphometrics · Olfaction · Elasmobranch · Teleost · X-ray tomography
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00429-020-02127-1) contains supplementary material, which is available to authorized users. * Victoria Camilieri‑Asch [email protected] 1
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Biosciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope, Stocker Road, Exeter EX4 4QD, UK
School of Biological Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
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Oceans Institute, Indian Ocean Marine Research Centre (IOMRC), The University of Western Australia, Cnr Fairway and Service Road 4, Crawley, WA 6009, Australia
Ocean Graduate School, IOMRC, The University of Western Australia, Cnr Fairway and Service Entrance 4, Craw
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