Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA
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RESEARCH ARTICLE
Open Access
Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA Ling Lin1, Yong-Song Yue1, Ni-Dan Wang1, Lei-Yan Wei1, Yang Han1, Xiao-Jing Song1, Zhi-Feng Qiu1, Wei Cao1 and Tai-Sheng Li1,2,3,4*
Abstract Background: A more time saving, convenient, reproducible, and scalable method is needed to assess total HIV-1 DNA levels. Methods: Frozen whole blood and peripheral blood mononuclear cell (PBMC) samples both 200 μl at the same point were used to detect total HIV-1 DNA. Automatic extraction of total HIV-1 DNA was used to ensure the consistency of sample extraction efficiency. The detection reagent was HIV-1 DNA quantitative detection kit and real-time quantitative PCR was utilized. Results: Of the 44 included patients, 42 were male and 2 were female, with a median age of 33 years. Thirtythree cases were collected after receiving antiviral treatment, with a median duration of treatment of 3 months, and the other 11 cases were collected before antiviral treatment. The median viral load was 1.83 log10 copies/mL, the median CD4 and CD8 count were 94 and 680 cells/μL, and the median CD4/CD8 ratio was 0.18. The results of the two samples were 3.02 ± 0.39 log10 copies/106 PBMCs in PBMC samples and 3.05 ± 0.40 log10 copies/106 PBMCs in whole blood samples. The detection results of the two methods were highly correlated and consistent by using paired t test (P = 0.370), pearson correlation (r = 0.887, P < 0.0001) and intra-group correlation coefficient (ICC = 0.887, P < 0.0001) and bland-altman [4.55% points were outside the 95% limits of agreement (− 0.340 ~ 0.390)]. Conclusions: The results of the whole blood sample test for total HIV-1 DNA are consistent with those of PBMC samples. In a clinical setting it is recommended to use whole blood samples directly for the evaluation of the HIV reservoir. Keywords: AIDS, HIV-1 DNA, Peripheral blood mononuclear cell, Reservoir, Whole blood
* Correspondence: [email protected] 1 Department of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, 1# Shuai Fu Yuan, Beijing 100730, China 2 Center for AIDS Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exce
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