QTL Mapping for Reproductive Maturity in Lowland Switchgrass Populations
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QTL Mapping for Reproductive Maturity in Lowland Switchgrass Populations Hongxu Dong 1 & Shuiyi Thames 1,3 & Linglong Liu 1,4 & Michael W. Smith 2 & Liuling Yan 1 & Yanqi Wu 1
# Springer Science+Business Media New York 2015
Abstract Switchgrass (Panicum virgatum L.) has potential to be a major cellulosic bioenergy crop. Selection for late flowering plants will extend the vegetative growth, likely resulting in larger biomass yields. However, the genetic structure for reproductive maturity in switchgrass is undefined. Accordingly, the major objective of this study was to identify genomic regions associated with reproductive development. Two lowland populations, one consisting of 176 progeny from NL94 (♀) × SL93 (♂) and a first-generation self-fertilized population of 265 progeny from NL94, were field established in a randomized complete block design with three replications at two Oklahoma locations in 2011. Phenotypic data of reproductive maturity in the populations were collected in 2012 and 2013. Significant genetic variation for reproductive maturity was observed within the two populations. Broad-sense heritabilities were 0.46 to 0.77 and 0.28 to 0.74 for the hybrid and selfed populations, respectively. A linkage map with 178 simple sequence repeat (SSR) markers of the hybrid population constructed in this study and a pre-existing linkage map of 439 Electronic supplementary material The online version of this article (doi:10.1007/s12155-015-9651-9) contains supplementary material, which is available to authorized users. * Yanqi Wu [email protected] 1
Department of Plant & Soil Sciences, Oklahoma State University, Stillwater, OK 74078, USA
2
Department of Horticulture and Landscape Architecture, Oklahoma State University, Stillwater, OK 74078, USA
3
Donald Danforth Plant Science Center, St. Louis, MO 63132, USA
4
National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
SSR markers in the selfed population were used for quantitative trait loci (QTL) characterization. QTL analyses revealed that reproductive maturity was controlled by multiple genomic regions. The QTL regions between nfsg-125 and PVE781/782 on linkage group (LG) 2b, between PVGA-1727/ 1728 and PVGA-1201/1202 on LG 3b, and between PVCA G-2503/2504 and PVAAG-3253/3254 on LG 7a were associated with reproductive maturity in both populations. The markers linked to the significant QTL could be used to accelerate the development of switchgrass germplasm with later flowering to increase biomass yield. Keywords Switchgrass . Simple sequence repeat . Reproductive maturity . QTL mapping
Introduction Switchgrass is a predominant tallgrass species of the North America prairies [1, 2]. Multiple merits of switchgrass make it a highly suitable herbaceous candidate for cellulosic feedstock production, including high biomass yield potential, adaptation to marginal lands, strong stress resistance, minimal requirement of agronomic inputs, stand
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