QTL mapping of resistance to Ug99 and other stem rust pathogen races in bread wheat
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QTL mapping of resistance to Ug99 and other stem rust pathogen races in bread wheat Silvina Baraibar & Richard García & Paula Silva & Bettina Lado & Ariel Castro & Lucía Gutiérrez & Monika Kavanová & Martín Quincke & Sridhar Bhavani & Mandeep S. Randhawa & Silvia Germán
Received: 26 June 2019 / Accepted: 23 October 2019 / Published online: 15 August 2020 # Springer Nature B.V. 2020
Abstract Most wheat cultivars planted worldwide are susceptible to the stem rust Ug99 race group. To prepare for the potential spread of these races into South America, we aimed to identify genomic S. Baraibar (*) : R. García : P. Silva : M. Kavanová : M. Quincke : S. Germán Instituto Nacional de Investigación Agropecuaria (INIA), Programa de Cultivos de Secano, Estación Experimental INIA La Estanzuela. Ruta 50, km 11, 70000 Colonia, Uruguay e-mail: [email protected] P. Silva Interdepartmental Genetics Program, Kansas State University, Manhattan, KS 66506, USA B. Lado Facultad de Agronomía, Universidad de la República, Garzón 780, 12900 Montevideo, Uruguay A. Castro Facultad de Agronomía, Universidad de la República, Estación Experimental Dr. Mario A. Cassinoni, Ruta 3, km 363, 60000 Paysandú, Uruguay L. Gutiérrez Department of Agronomy, University of Wisconsin–Madison, 1575, Linden Dr, Madison, WI 53706, USA S. Bhavani International Maize and Wheat Improvement Center (CIMMYT), Km. 45, Carretera, México-Veracruz, El Batán, CP 56237 Texcoco, Edo. de México, Mexico M. S. Randhawa International Maize and Wheat Improvement Center (CIMMYT), World Agroforestry Centre (ICRAF), United Nations Avenue, Gigiri, P.O. Box 1041, Nairobi 00621, Kenya
regions responsible for resistance to Ug99 race group in germplasm adapted to South America. Two RIL populations from a cross between a stem rust susceptible parent “Baguette 13” and resistant local parents “INIA Tero” and “BR23//CEP19/PF85490” were developed. Phenotypical evaluation was completed at the seedling stage in Uruguay and under field conditions in Uruguay and Kenya. Both RIL populations were genotyped using the GBS approach. Besides Sr24, three other resistance loci in “INIA Tero” were detected on chromosomes 2B, 6A, and 7B. All four QTL were effective to local races, whereas only the QTL on chromosome 2B was effective against the Ug99 race group. Besides Sr31, “BR23//CEP19/ PF85490” also carries two other stem rust resistance loci on chromosomes 2B and 6A. All three explained the resistance in Uruguay, while only the QTL on 2B was effective to Ug99 in Kenya. The physical location suggested that the QTL identified on chromosome 2B in both populations may correspond to Sr28, which was confirmed using specific molecular markers. Further studies are needed to determine the relationship between QTL for resistance to local races identified on chromosomes 6A and 7B and previously reported resistance genes and QTL. The results of this study are highly relevant for breeding wheat cultivars with diverse and durable resistance to stem rust.
Keywords Triticum aestivum . QTL mapping . Genetic resis
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