Quantitative Identification and Antioxidant Activity In Vitro of Phenolic Compounds from the Old Leaves of Toona sinensi
In the present study, HPLC methodogy was developed for the simultaneously determining the major phenolic compounds such as rutin, gallic acid, quercetin, and kaempferol in the old leaves of Toona sinensis. The results showed that the percentage contents o
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Quantitative Identification and Antioxidant Activity In Vitro of Phenolic Compounds from the Old Leaves of Toona sinensis Changjin Liu, Hongying Wan, Jie Zhang and Zetian Hua Abstract In the present study, HPLC methodogy was developed for the simultaneously determining the major phenolic compounds such as rutin, gallic acid, quercetin, and kaempferol in the old leaves of Toona sinensis. The results showed that the percentage contents of rutin, gallic acid, quercetin, and kaempferol were (1.55 ± 0.01) %, (0.87 ± 0.02) %, (2.27 ± 0.05) %, and (1.31 ± 0.01) % from purified phenolics of Toona sinensis old leaves respectively. Antioxidation activity of Toona sinensis phenolics before purified and after purified were studied, the results indicated that the purified phenolics in Toona sinensis old leaves had a significant capacity for scavenging DPPH free radical and superoxide anion radical but less effective on scavenging hydroxyl radical. The phenolics existed in Toona sinensis leaves showed obvious antioxidant effect on soybean oil, which could be as an alternative natural antioxidant. Keywords Toona sinensis
Phenolic compounds HPLC Antioxidation
54.1 Introduction Toona sinensis (TS) Roemor, a perennial deciduous tree also called ‘‘Xiangchun,’’ is a kind of native plant in China. It has been cultivated for more than 2300 years [1]. The tender leaves and stem have been used for the treatment of enteritis, carminative, itch, and dysentery in the oriental medicine [2]. Its young leaves can C. Liu H. Wan (&) J. Zhang Z. Hua Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China e-mail: [email protected] C. Liu Z. Hua China National Japonica Rice Research and Development Center, Tianjin 300457, People’s Republic of China
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 249, DOI: 10.1007/978-3-642-37916-1_54, Ó Springer-Verlag Berlin Heidelberg 2014
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be used as a kind of vegetable called ‘‘Xiangchun Ya’’ in China and thus is known as a ‘‘tree Vegetable’’ [3]. The edible leaves have been used as an oriental medicine for treating dysentery, rheumatoid arthritis, enteritis, gastric ulcers, itchiness, and cancer [4]. Various biological activities of TS leaf extracts have been reported, including antioxidant effects [5], anti-cancer [6], and anti-diabetes [7] as well as the suppressing brain degeneration in senescence-accelerated mice [8] and inhibiting Leydig cell steroidogenesis [9]. Meanwhile, several methods have been employed for the analysis of ingredients in TS. Gradient reversed-phase high performance liquid chromatography (HPLC), 1HPLC-mass, CE-ED [10], and thinlayer chromatography (TLC) are the most powerful analytical methods [11]. Antioxidant compounds extract from the plant extract antioxidant compounds potentiate body’s antioxidant defense or act as antioxidant and
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