Quantitative Proteomics by Mass Spectrometry
This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand manner with many
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Salvatore Sechi Editor
Quantitative Proteomics by Mass Spectrometry Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Quantitative Proteomics by Mass Spectrometry Second Edition
Edited by
Salvatore Sechi NIDDK, National Institutes of Health, Bethesda, MD, USA
Editor Salvatore Sechi NIDDK, National Institutes of Health Bethesda, MD, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3522-2 ISBN 978-1-4939-3524-6 (eBook) DOI 10.1007/978-1-4939-3524-6 Library of Congress Control Number: 2016931330 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2007, 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover image: This image is composed of 4 elements: A 3D image of a cell where the nucleus is visible, a double helix DNA that comes out of the nucleus and joins/morphs into a 3D ribbon structure of a protein. On top of these 3 elements there is a Mass Spectra. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Tools for Next Generation Quantitative Proteomics by Mass Spectrometry The proteome has been defined as the entire set of proteins expressed by a genome. The genome is relatively simple, and using today’s technology the human genome of an individual can be routinely sequenced. The dynamic nature of the proteome, and its heterogeneous structure with many possible post-translational modifications, make the task of fully sequencing the complete human proteome out of reach. On one hand, “gene-centric” proteomic approaches that focus on identifying the genes that code for the expressed proteins (which is distinct from characterizing the complete molecular sequences of the protein
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