Quantitative Methods in Proteomics
Protein modifications and changes made to them, as well as the quantities of expressed proteins, can define the various functional stages of the cell. Accordingly, perturbations can lead to various diseases and disorders. As a result, it has become paramo
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Quantitative Methods in Proteomics Edited by
Katrin Marcus Department of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany
Editor Katrin Marcus Department of Functional Proteomics Medizinisches Proteom-Center Ruhr-Universität Bochum Universitätsstraße 150 44801 Bochum Germany
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-884-9 e-ISBN 978-1-61779-885-6 DOI 10.1007/978-1-61779-885-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012937651 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Protein modifications and changes to them as well as the quantities of expressed proteins can define the various functional stages of the cell. Accordingly, perturbations can lead to various diseases and disorders. Consequently, it became paramount to be able to detect and monitor posttranslational modifications and to measure the abundance of proteins within the cell with extreme sensitivity. Thus, protein analysis has acquired an importance that goes well beyond protein identification per se. Today, protein identification is an almost routine requirement. However, reliable techniques for quantifying unmodified proteins (including those that escape detection under standard conditions, such as protein isoforms and membrane proteins) as well as the detection and quantification of posttranslational protein modifications are still far from being a matter of routine. Hence, there is a need for a profound understanding of the principles underlying modern protein analysis, in order to apply and improve established and novel methods successfully. This book gives a detailed survey of “Quantitative Methods in Proteomics” addressing topics and methods from statistical issues when planning proteomics experiments (Chapter 1), gel-based (Chapters 2–6) to mass spectrometry-based applications including relative and absolute quantification using enzymatic, chemical, and metabolic labeling as well as label-free approaches (Chapte
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