Rapid and sensitive fluorescence and smartphone dual-mode detection of dopamine based on nitrogen-boron co-doped carbon
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ORIGINAL PAPER
Rapid and sensitive fluorescence and smartphone dual-mode detection of dopamine based on nitrogen-boron co-doped carbon quantum dots Sahar Dadkhah 1 & Ali Mehdinia 2
&
Ali Jabbari 1 & Ahmad Manbohi 2
Received: 28 December 2019 / Accepted: 2 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A dual-mode fluorescence and colorimetric biosensor based on nitrogen-boron co-doped carbon quantum dot (N-B CQDs) for rapid and sensitive detection of dopamine (DA) was developed. The quantum dot luminescent materials, N-B CQDs, were prepared by a one-step microwave-assisted method. The N-B CQDs were characterized using SEM, HR-TEM, XRD, FT-IR, Raman, fluorescence, and UV-Vis techniques. The dual-mode assays of fluorescence and colorimetric methods were used for detection of DA. The high fluorescent N-B CQDs mediated turn-off assay for the facile room temperature detection of dopamine via inner filter effect (IFE) and Forster resonance energy transfer (FRET) processes at basic pH. The colorimetric detection of DA was also developed via in-house android application using a smartphone and N-B CQD solution-based nanosensor. The smartphone-based colorimetric biosensors generated more reliable information for quantitative analysis of color changes than the naked eye. Furthermore, a smartphone application with N-B CQD solution-based nanosensor was integrated to monitor the color changes through the DA addition. Wide linear ranges were achieved for DA in the ranges 0.25–50 μM and 5–500 μM with fluorescence and smartphonebased method, respectively. The satisfactory results of the dual-mode detection of DA, not only in aqueous solution, but also in human urine and serum biological sample demonstrated its potential application in biosensing, as a point of care diagnostic tool. Keywords Dual-mode nanosensor . Quantum dots . Fluorescence detection . Colorimetric detection . Smartphone-based biosensor . Dopamine
Introduction In recent years, dual-mode sensors have attracted much attention and become an effective strategy in point of care (POC) diagnostic tool, food safety, and environmental monitoring. Compared with single-mode approach, the dual-mode assays such as fluorescence/colorimetry [32, 40], surface-enhanced Raman scattering (SERS)/fluorescence [48], and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04543-w) contains supplementary material, which is available to authorized users. * Ali Mehdinia [email protected] 1
Department of Chemistry, Faculty of Science, K. N. Toosi University of Technology, Tehran, Iran
2
Iranian National Institute for Oceanography and Atmospheric Science, Tehran, Iran
fluorescence/imaging have been shown to be more sensitive and efficient. Beside the individual advantages of each method used in dual-mode detection, this mode provides a double check readout approach. Therefore, a more reliable and accurate quantitative analysis, especially in biologically complicated sample, can be performed
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