Re-identifying Grateloupia yangjiangensis (Rhodophyta, Halymeniaceae) based on morphological observations, life history
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Re-identifying Grateloupia yangjiangensis (Rhodophyta, Halymeniaceae) based on morphological observations, life history and rbcL sequence analyses WANG Hongwei1*, GUO Shaoru1, ZHANG Xiaoming1, ZHAO Dan1, ZHANG Wen1, LUAN Rixiao2 1 College 2
of Life Sciences, Liaoning Normal University, Dalian 116081, China Dalian Natural History Museum, Dalian 116023, China
Received 31 August 2012; accepted 28 January 2013 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2014
Abstract On the basis of morphological observations, life history and molecular phylogeny, Grateloupia yangjiangensis, which is similar to G. filicina, G. orientalis, G. catenata, and G. ramosissima in appearance, was re-examined. The results are as follows: (1) the auxiliary-cell ampullae of G. yangjiangensis were of Grateloupia type, thalli was fleshy and gelatinous in texture, and the erect axes were compressed; the cortex was 0.25–0.30 mm thick, consisting of five to seven outer layers, and there were five inner layers of triangular or stellate cells; (2) there was no filamentous stage in the development of the carpospores; (3) the ribulose-1,5-bisphosphate carboxylase/oxygenase gene (rbcL) sequence of four G. yangjiangensis examined showed that there was no intergeneric divergence among them, and for the phylogenetic tree, four sequences of G. yangjiangensis formed a single monophyletic subclade within the large Grateloupia clade of Halymeniaceae. In conclusion, G. yangjiangensis was a single species within the genus Grateloupia. This research provided criterion for identification and cultivation of G. yangjiangensis. Key words: Grateloupia yangjiangensis, Halymeniaceae, Rhodophyta, morphological observations, life history, rbcL Citation: Wang Hongwei, Guo Shaoru, Zhang Xiaoming, Zhao Dan, Zhang Wen, Luan Rixiao. 2014. Re-identifying Grateloupia yangjiangensis (Rhodophyta, Halymeniaceae) based on morphological observations, life history and rbcL sequence analyses. Acta Oceanologica Sinica, 33(4): 77–84, doi: 10.1007/s13131-014-0450-5
1 Introduction Grateloupia yangjiangensis Li et Ding within the family Halymeniaceae of Rhodophyta was initially identified on the base of external morphological features, transverse view and tetrasporangia. The species was first recorded in Flora Algarum Marinarum Sinicarum (Xia, 2004) and has not been reported in any published papers. Furthermore, no descriptions currently exist about the reproductive structures and development of G. yangjiangensis sporelings. Spore characteristics such as development type, form, and size can be used for classification of red algae (Shunpei, 1947). Spore development of most Grateloupia species was found to be “indirect discal type”, with filamentous fronds sometimes appearing during early sporeling development (Shunpei, 1947). Sporogenesis modes of G. filicina collected from Xiamen and G. ramosissima were “indirect discal type”; however, the germinal form of their initial stage differed, which may be considered as a difference in species (Liu and Li, 1986). Filam
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