Rearing in intermediate salinity enhances immunity and disease-resistance of turbot ( Scophthalmus maximus L.)
- PDF / 142,027 Bytes
- 7 Pages / 595 x 842 pts (A4) Page_size
- 86 Downloads / 212 Views
Rearing in intermediate salinity enhances immunity and disease-resistance of turbot (Scophthalmus maximus L.) ZHANG Yanjiao1 , MAI Kangsen1∗ , MA Hongming1 , AI Qinghui1 , ZHANG Wenbing1 , XU Wei1 1
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China
Received 29 December 2009; accepted 2 June 2010 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2011
Abstract Studies were conducted to investigate the non-specific immune responses and disease-resistance of juvenile turbot Scophthalmus maximus, cultured at four different salinities (8, 20, 32 and 40). Three concentrations (3.75×107 , 3.75×108 and 3.75×109 CFU/ml) of Vibrio anguillarum suspension were employed at each salinity to determine the 4-day LD50 . The serum lysozyme activity, the alternative complement pathway activity (ACH50) and the phagocytosis percentage of head kidney in turbot were tested at 24, 48 and 72 h post-challenge of V. anguillarum (1.1×108 CFU/ml, 0.1 ml), respectively, to evaluate the non-specific immune responses at the selected rearing salinities. Fish reared at salinity 20 had the lowest mortality, namely, the highest 4-day LD50 value (8.88±0.17). Besides, the lysozyme activity, ACH50 and the phagocytosis of turbot were the highest at the salinity 20, but with the lowest at the salinity 40 treatment regardless of sampling time. In addition, the non-specific immune activities kept increasing within 72 h post-challenge of V. anguillarum, except that the lysozyme activity increased from 24 to 48 h, and then decreased from 48 to 72 h at 40 significantly. These results together indicate that rearing in intermediate salinity (20) was able to enhance the immunity and disease-resistance of turbot. Key words: Scophthalmus maximus, salinity, immunity, disease-resistance, Vibrio anguillarum
tains anatomical, humoral and cellular barriers that constitutes the first defense line against pathogens in fish and is normally efficient to protect it from infections until specific immune response (Ellis, 2001; Jones, 2001). Environmental factors are in associated with the occurrence of diseases. Usually, the environmental conditions deteriorate before the diseases occur. Many studies have shown that the environmental factors, such as water temperature (Alcorn et al., 2002; Martinez-Palacios et al., 2002) and salinity (Partridge and Jenkins, 2002) affect the growth, survival and disease-resistance of aquatic animals. In fish, the studies of the effects of salinity have mainly been focused on the changes in osmoregulations, plasma parameters, energy metabolism and growth (McCormick, 2001; McCormick, 1995; Maetz, 1974). It was demonstrated that the grouper fry (Epinephelus sp.) exposed to either hypo or hyperosmotic shock increased their susceptibility to infectious pancreatic necrosis virus
1 Introduction Turbot (Scophthalmus maximus L.) is one of the most commercially important fish in mariculture, which is distributed along the coast of Europe from the Mediterranean Sea to the Norway Sea. It w
Data Loading...
