Recent advances in microfluidic technologies for separation of biological cells
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Recent advances in microfluidic technologies for separation of biological cells Lujing Sun 1 & Wenguang Yang 1 Lianqing Liu 2,3
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Shuxiang Cai 1 & Yibao Chen 1 & Honghui Chu 1 & Haibo Yu 2,3 & Yuechao Wang 2,3 &
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Cell separation has always been a key topic in academic research, especially in the fields of medicine and biology, due to its significance in diagnosis and treatment. Accurate, high-throughput and non-invasive separation of individual cells is key to driving the development of biomedicine and cellular biology. In recent years, a series of researches on the use of microfluidic technologies for cell separation have been conducted to solve bio-related problems. Hence, we present here a comprehensive review on the recent developments of microfluidic technologies for cell separation. In this review, we discuss several cell separation methods, mainly including: physical and biochemical method, their working principles as well as their practical applications. We also analyze the advantages and disadvantages of each method in detail. In addition, the current challenges and future prospects of microfluidic-based cell separation were discussed. Keywords Microfluidic technology . Cell separation
1 Introduction Cell separation has always been a problem facing researchers in the fields of medicine and biology(xiang et al. 2019; Bacon et al. 2020; Nagase et al. 2020). It is of great importance in biomedical research, including diagnosis and cellular biology. For instance, some cells are found at extremely low numbers in the blood, such as circulating tumor cells (CTCs), fetal nucleated red blood cells (NRBCs), circulating endothelial cells, immune cells subgroup, and stem cells(Myung et al. 2010; Li et al. 2015; Jones and Watt 1993; Tayoun et al. 2019; Safarpour et al. 2020). Separation and investigation of these cells can help with the diagnosis of certain diseases. For example, CTCs are tumor cells that spread into peripheral * Wenguang Yang [email protected] * Lianqing Liu [email protected] 1
School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China
2
State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
3
Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
blood and circulate with the blood due to primary focus or metastasis of solid tumors shed into blood. Studies have shown that CTCs can lead to the occurrence of tumor metastasis(Tanaka et al. 2009; Martin et al. 2017; Kim et al. 2019; Gupta and Massagué 2006; Rengan et al. 2015). The effective capture of CTCs and the detection of their genes and secretions offer important guidance for the early diagnosis, postoperative evaluation, personalized treatment and evaluation of tumor resistance. NRBCs play an important role in prenatal diagnosis(Kumo et al. 2017; Wei et al. 2019; Antfolk and Laurell 2017). NRBCs contain complete g
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