Repeated centrifuging and washing concentrates bacterial samples in peritoneal dialysis for optimal culture: an original
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RESEARCH ARTICLE
Open Access
Repeated centrifuging and washing concentrates bacterial samples in peritoneal dialysis for optimal culture: an original article Ni Tien1,2, Bang-Jau You3,4, Hsuan-Jen Lin5, Chieh-Ying Chang6, Che-Yi Chou5, Hsiu-Shen Lin1,2, Chiz-Tzung Chang7,8*, Charles C. N. Wang9,10 and Hung-Chih Chen9,11*
Abstract Background: Bacterial cultures allow the identification of infectious disease pathogens. However, obtaining the results of conventional culture methods is time-consuming, taking at least two days. A more efficient alternative is the use of concentrated bacterial samples to accelerate culture growth. Our study focuses on the development of a high-yield sample concentrating technique. Results: A total of 71 paired samples were obtained from patients on peritoneal dialysis (PD). The peritoneal dialysates were repeat-centrifuged and then washed with saline, namely the centrifuging and washing method (C&W method). The concentrated samples were Gram-stained and inoculated into culture plates. The equivalent unprocessed dialysates were cultured as the reference method. The times until culture results for the two methods were compared. The reference method yielded no positive Gram stain results, but the C&W method immediately gave positive Gram stain results for 28 samples (p < 0.001). The culture-negative rate was lower in the C&W method (5/71) than in the reference method (13/71) (p = 0.044). The average time for bacterial identification achieved with the C&W method (22.0 h) was shorter compared to using the reference method (72.5 h) (p < 0.001). Conclusions: The C&W method successfully concentrated bacterial samples and superseded blood culture bottles for developing adequate bacterial cultures. The C&W method may decrease the culture report time, thus improving the treatment of infectious diseases. Keywords: Repeat centrifuging and washing, Peritonitis, Peritoneal dialysis, Bacterial culture
Background The current method for culturing samples of infected body fluids involves inoculating the specimen into a blood culture bottle and then incubating the sample within an automated culture machine [1, 2]. After the machine automatically detects bacterial signals within * Correspondence: [email protected]; [email protected] 7 College of Medicine, China Medical University, Taichung, Taiwan 9 Department of Bioinformatics and Medical Engineering Asia University, Taichung 41354, Taiwan Full list of author information is available at the end of the article
the blood culture bottle, the broth within the blood culture bottle is streaked onto culture plates containing different culture media for further culture. Biochemical methods can identify bacterial colonies, which are subsequently isolated. One example of such a method is matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) [3–5]. After bacterial identification, the antibiotic susceptibility test is performed using the disc diffusion of automated antimicrobial susceptibility testing systems [6]. This
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