Research on promoting liver fibrosis injury by the targeted regulation of miR-202 for HGF to activate HSC
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ORIGINAL ARTICLE
Research on promoting liver fibrosis injury by the targeted regulation of miR-202 for HGF to activate HSC Xianli Niu 1,2 & Shirong Nong 1 & Junyuan Gong 1 & Xin Zhang 1 & Hui Tang 1 & Tianhong Zhou 1 & Wei Li 1 Received: 18 June 2019 / Accepted: 7 March 2020 # Royal Academy of Medicine in Ireland 2020
Abstract Background Liver fibrosis is the primary cause of liver cirrhosis and hepatocellular carcinoma and leads to considerable morbidity and mortality. Recent studies have shown that microRNAs are associated with fibrotic processes in liver disorders, but the exact role of miR-202 is still unclear, and its related mechanisms are not fully understood. Aims The aim of this research is to analyze the regarded regulation of miR-202 on HGF and its role in the pathological progress of liver fibrosis. Methods In the present study, qRT-PCR was used to detect the expression level of miR-202 in serum of patients with liver fibrosis and to compare its expression in patients with different pathological stages. HGF was predicted to be the target gene of miR-202 by TargetScan and was verified by Dual-luciferase reporter gene assay. qRT-PCR and western blot were used to detect the regulatory effect of mir-202 on the mRNA and protein of HGF; effect of miR-202 on the expression of fibrosis factors αsmooth muscle actin (α-SMA), FSP1, and collagen was detected; effect of miR-202 on liver fibrosis in mice was detected by establishing CCL4-induced mouse model. Results We found that the expression level of miR-202 in serum of patients with liver fibrosis was significantly higher than that of healthy people, and increased with the increase of fibrosis; miR-202 inhibited the expression level of mRNA and protein of HGF by combining with the 3’-UTR of HGF; the expression level of miR-202 significantly increased after hepatic stellate cells (HSC) were stimulated by AngII; the overexpression of miR-202 could up-regulate the expression of fibrotic factors α-SMA, FSP1, and collagen I. In addition, miR-202 up-regulated the expression of collagen I and collagen III in liver tissue of mice with liver fibrosis and promoted the progress of liver fibrosis. Conclusions miR-202 could negatively regulate the expression of target gene HGF, activated HSC, and increased the expression levels of various fibrosis factors, and the pathological process of liver fibrosis injury was promoted. Keywords HGF . HSC, liver fibrosis . miR-202
Introduction Liver fibrosis is a kind of excessive tissue repair that occurs in the development of various chronic liver injury diseases, including chronic viral hepatitis, alcoholic hepatitis, autoimmune liver diseases, as well as liver injury caused by parasites,
* Wei Li [email protected] 1
Key Laboratory of Viral Biology Guangzhou, Department of Biology, Jinan University, No.601, West Huangpu Avenue, Guangzhou 510632, Guangdong, China
2
Department of Biochemistry and Molecular Biology, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong, China
metabolic diseases, poisons, drugs, etc. [1, 2]
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