Responses of Ammonia-Oxidizing Archaea and Bacteria in Malodorous River Sediments to Different Remediation Techniques
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MICROBIOLOGY OF AQUATIC SYSTEMS
Responses of Ammonia-Oxidizing Archaea and Bacteria in Malodorous River Sediments to Different Remediation Techniques Yan He 1
&
Yunchang Zhou 1 & Rui Weng 1 & Jianhua Wang 1 & Jinghan Chen 1 & Minsheng Huang 1
Received: 31 May 2020 / Accepted: 1 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In this study, the joint use of high throughput sequencing, real-time quantitative PCR, and ammonia-oxidizing bacteria (AOB)– inhibiting allylthiourea was used to differentiate between the contributions of ammonia-oxidizing archaea (AOA) vs AOB to ammonia oxidation and ascertain how AOA and AOB responded to two widely used river remediation techniques (aeration and Ca(NO3)2 injection). Results showed that ammonia oxidation was largely attributed to ATU-sensitive AOB rather than AOA and Nitrosomonas was the predominant AOB-related genus (53.86%) in the malodorous river. The contribution of AOB to ammonia oxidation in the context of aeration and Ca(NO3)2 injection was 75.51 ± 2.77% and 60.19 ± 10.44%, respectively. The peak of AOB/AOA ratio and the marked increase of relative abundances of Nitrosomonas and Nitrosospira in aeration runs further demonstrated aeration favored the ammonia oxidation of AOB. Comparatively, Ca(NO3)2 injection could increase the ammonia oxidation contribution of AOA from 31.32 ± 6.06 to 39.81 ± 10.44% and was significantly correlated with Nitrosococcus of AOB (r = 0.796, p < 0.05), Candidatus_Nitrosopelagicus of AOA (r = 0.986, p < 0.01), and AOA Simpson diversity (r = − 0.791, p < 0.05). Moreover, Candidatus_Nitrosopelagicus was only present in Ca(NO3)2 runs. Taken together, Ca(NO3)2 was recognized as an important factor in mediating the growth and ecological niches of ammonia oxidizers. Keywords Malodorous river sediment . Ammonia-oxidizing archaea . Ammonia-oxidizing bacteria . Aeration . Calcium nitrate injection
Introduction Oxidation of ammonia to nitrite is an integrative part in nitrogen cycling and is mediated by two distinct groups of
microorganisms, i.e., ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) [1]. Due to the physiological and metabolic differences of these two kinds of ammonia oxidizers, their distribution and relative contribution to
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00248-020-01597-4) contains supplementary material, which is available to authorized users. * Yan He [email protected] Yunchang Zhou [email protected] Rui Weng [email protected] Jianhua Wang [email protected] Jinghan Chen [email protected]
Minsheng Huang [email protected]
1
Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, Institute of Eco-Chongming, Technology Innovation Center for Land Spatial Eco-restoration in Metropolitan Area, Ministry of Natural Resources, East China Normal University, S
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