Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms: A Fluorescence Microscopy Approach
Reactive oxygen species (ROS) production within biofilms is studied with a simple and easy setup based on fluorescence microscopy. Herein, a biofilm is exposed to different ROS inducers: a bactericidal antibiotic (ciprofloxacin) and a BODIPY-based photose
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Jesús Espada Editor
Reactive Oxygen Species Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes: http://www.springer.com/series/7651
Reactive Oxygen Species Methods and Protocols
Edited by
Jesús Espada Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, Madrid, Spain; Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O’Higgins, Santiago, Chile
Editor Jesús Espada Ramón y Cajal Institute for Health Research (IRYCIS) Ramón y Cajal University Hospital Madrid, Spain Centro Integrativo de Biología y Química Aplicada (CIBQA) Universidad Bernardo O’Higgins Santiago, Chile
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-0716-0895-1 ISBN 978-1-0716-0896-8 (eBook) https://doi.org/10.1007/978-1-0716-0896-8 © Springer Science+Business Media, LLC, part of Springer Nature 2021 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, expressed or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 1 New York Plaza, New York, NY 10004, U.S.A.
Preface The signaling properties of reactive oxygen species (ROS) in biological systems under physiological conditions are currently a hot and exciting area of research. For one side, ROS are a major consequence of the aerobic metabolism that can turn out as extremely harmful for most living organisms. Not surprisingly, an aberrant production of ROS in mammalian tissues has been associated with several critical diseases, including cancer, and with the aging process. However, different ROS-based molecular signaling mechanisms are being steadily characterized in different experiment
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