Rhizoextraction Potential of Convolvulus tricolor Hairy Roots for Cr 6+ , Ni 2+ , and Pb 2+ Removal from Aqueous Solutio
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Rhizoextraction Potential of Convolvulus tricolor Hairy Roots for Cr6+, Ni2+, and Pb2+ Removal from Aqueous Solutions Kateryna Lystvan 1
2
1
& Vitalii Listvan & Natalia Shcherbak & Mykola Kuchuk
1
Received: 5 May 2020 / Accepted: 9 November 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
This study evaluated the potential of dwarf morning-glory Convolvulus tricolor (Convolvulaceae) plants and their hairy roots induced by Agrobacterium rhizogenes for rhizoextraction of heavy metals ions from the liquid medium under aseptic growth conditions. Both the young C. tricolor plants and the generated hairy root culture efficiently removed Cr6+, Ni2+, and Pb2+ ions from the liquid cultivation medium. As determined by atomic absorption spectroscopy, the hairy roots demonstrated a high level of heavy metal ions accumulation (μg/g dry weight): 3942 ± 210 of chromium, 1529 ± 312 of nickel, and 2613 ± 373 of lead. These data show that the hairy roots of morning glory might be of interest for some phytoremediation strategies due to their high bioaccumulation abilities. The comparison of bioaccumulation potential of established hairy roots and young C. tricolor plants give grounds to suppose that roots of C. tricolor play an active role in the absorption of Cr6+, Ni2+, and Pb2+ from liquid media, whereas the aboveground part rather serves as a storage for the accumulated metal ions. Keywords Convolvulustricolor . Rhizoextraction . Convolvulaceae . Hairyroots . Invitroculture . Heavy metals
Introduction Water pollution by various organic and inorganic harmful substances resulting from human activity has aroused as a serious environmental problem. The widespread use of heavy metal salts in different industries (textile, galvanizing, tannery, etc.) generates large quantities of
* Kateryna Lystvan [email protected]
1
Department of Genetic Engineering, Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Acad.Zabolotnoho str, 148, Kyiv 03143, Ukraine
2
Zhytomyr Ivan Franko State University, Zhytomyr, Ukraine
Applied Biochemistry and Biotechnology
toxic liquid wastes containing high amounts of heavy metals. Rhizofiltration, the absorption and accumulation of toxic substances by plant roots, was developed as one of the methods for water decontamination [1]. In addition to the absorption of various contaminants, roots can excrete different substances capable of interacting chemically or enzymatically with the pollutants and neutralizing them. The phytoremediation ability of various plant species has been actively investigated, with more recent attempts to increase phytoremediation potential using in vitro culture and transgenic manipulations [2]. Exploring plant root culture, including transgenically induced “hairy roots”, is one of the directions of such investigations [3–6]. High efficiency of heavy metal uptake by hairy root cultures has been demonstrated for some plant species, including Thlaspi caerulescens, Brassica juncea, and some Alyssum species [7–10]. Hairy root
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