RNA Aptamers: A Review of Recent Trends and Applications
RNA aptamers, small oligonucleotides derived by an in-vitro selection process called SELEX (Systematic Evolution of Ligands by EXperimental enrichment), are important candidates for therapeutic and diagnostic applications. RNA aptamers have high affinity
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RNA Aptamers: A Review of Recent Trends and Applications Kyung-Nam Kang and Yoon-Sik Lee Abstract RNA aptamers, small oligonucleotides derived by an in-vitro selection process called SELEX (Systematic Evolution of Ligands by EXperimental enrichment), are important candidates for therapeutic and diagnostic applications. RNA aptamers have high affinity and specificity for their target molecules. In this review, we describe methods for generating RNA aptamers (the SELEX technique and modified SELEX processes) and therapeutic applications for diseases such as neovascular age-related macular degeneration (AMD), inflammatory diseases, and obesity. We also analyze the social networks among researchers and organizations (universities, research institutes, firms, etc.) that are active in the pursuit of aptamer-based therapeutic approaches. This study provides relevant information on recent research trends in RNA aptamers. Keywords RNA aptamer
Therapeutic application Social networks
Abbreviations CAD CNV MCP-1 NIH OECD PCI PEG RADAR
Coronary artery disease Choroidal neovascularization Monocyte chemoattractant Protein 1 National Institutes of Health Organization for Economic Cooperation and Development Percutaneous coronary intervention Polyethylene glycol A Randomized, partially-blinded, multi-center, active-controlled, doseranging study assessing the safety, efficacy, and pharmacodynamics
K.-N. Kang Korea Institute of Intellectual Property, KIPS Center, 9th FL. 647-9, Yeoksam-dong, Gangnam-gu, Seoul 135-980, Korea e-mail: [email protected] Y.-S. Lee (&) School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Korea e-mail: [email protected]
K.-N. Kang and Y.-S. Lee
SDF-1 SELEX SMEs VEGF
of the REG1 anticoagulation system compared to unfractionated heparin or low molecular heparin in subjects with acute coronary syndromes Stromal cell-derived factor-1 Systematic evolution of ligands by exponential enrichment Small- and medium-sized enterprises Vascular endothelial growth factor
Contents 1 2
Introduction.............................................................................................................................. Methods for Generating RNA Aptamers................................................................................ 2.1 The SELEX Method ....................................................................................................... 2.2 Modified SELEX ............................................................................................................ 3 Applications of RNA Aptamers.............................................................................................. 3.1 Therapeutic Approaches ................................................................................................. 3.2 Diagnostic Approaches ................................................................................................... 4 Social Networks in Therapeutic Applications of RNA Aptamers ........................................ 4.1 Discovery and Devel
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