RNA Detection Methods and Protocols
This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of
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Imre Gaspar Editor
RNA Detection Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
RNA Detection Methods and Protocols
Edited by
Imre Gaspar Developmental Biology Unit, EMBL Heidelberg, Heidelberg, Germany
Editor Imre Gaspar Developmental Biology Unit EMBL Heidelberg Heidelberg, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7212-8 ISBN 978-1-4939-7213-5 (eBook) DOI 10.1007/978-1-4939-7213-5 Library of Congress Control Number: 2017957614 © Springer Science+Business Media LLC 2018 Open Access Chapter 10 is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). For further details see license information in the chapter. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover Caption: Localization of oskar mRNA (in cyan, labelled with a set of FIT probes) to the posterior pole of a developing Drosophila oocyte (on the right). oskar mRNA is transcribed in the nurse cells (on the left) and transported through cytoplasmic bridges into the oocyte by cytoplasmic dynein. In the oocyte, localization of oskar to the posterior pole is mediated by kinesin heavy chain (in red, labelled with mKate2 fluorescent protein). Note, that oskar mRNA is exclusively expressed in the female germ-line (nurse cells and the oocyte) and it is absent from the somatic follicular epithelium (on the right, marked by kinesin) surrounding the oocyte. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York The registered company address is: 233 Spring Street, New York, NY 10
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