RNA Methylation Methods and Protocols
This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspect
- PDF / 7,332,884 Bytes
- 299 Pages / 504.63 x 737.01 pts Page_size
- 76 Downloads / 214 Views
Alexandra Lusser Editor
RNA Methylation Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
RNA Methylation Methods and Protocols
Edited by
Alexandra Lusser Division of Molecular Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria
Editor Alexandra Lusser Division of Molecular Biology Biocenter, Medical University of Innsbruck Innsbruck, Austria
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6805-3 ISBN 978-1-4939-6807-7 (eBook) DOI 10.1007/978-1-4939-6807-7 Library of Congress Control Number: 2017933288 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The aim of this book is to provide a comprehensive compendium of current methods and protocols to study posttranscriptional base modifications, in particular methylation, in RNA. The study of RNA methylation has emerged as an exciting new research area over the past few years not least due to the discovery that internal base methylation, such as N6-methyladenosine (m6A) and 5-methylcytosine (m5C), is widespread not only in abundant RNAs (tRNAs, rRNA) but also in mRNAs and in long noncoding RNAs (lncRNAs). Thus, it might represent an additional layer of gene regulation, leading to the coining of the terms “RNA epigenetics” and “epitranscriptomics.” Although RNA methylation research is still in its early stages, disruption of RNA methylation has been linked to a number of dise
Data Loading...
