RNA Vaccines Methods and Protocols
This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinic
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Thomas Kramps · Knut Elbers Editors
RNA Vaccines Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
RNA Vaccines Methods and Protocols
Edited by
Thomas Kramps Boehringer Ingelheim Pharma GmbH & Co. KG, Ingelheim am Rhein, Germany
Knut Elbers Boehringer Ingelheim GmbH, Ingelheim am Rhein, Germany
Editors Thomas Kramps Boehringer Ingelheim Pharma GmbH & Co. KG Ingelheim am Rhein, Germany
Knut Elbers Boehringer Ingelheim GmbH Ingelheim am Rhein, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6479-6 ISBN 978-1-4939-6481-9 (eBook) DOI 10.1007/978-1-4939-6481-9 Library of Congress Control Number: 2016958318 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Vaccines are currently regaining attention from members of the medical and scientific communities but even the broader public, including heads of state. This level of public awareness of the fundamental relevance of vaccines for global human well-being has been rekindled by dramatic threats of rapidly emerging infectious diseases (predominantly caused by viruses) and increasingly widespread multidrug-resistant bacterial infections. Insect- borne Zika virus and Ebola fever are only the most recent examples demonstrating a persistent vulnerability of human society to such primordial threats. In another area, cancer immunotherapy, vaccines are a promising, innovative treatment modality, too. In future, integrated treatment regimens that include cancer vaccines may enable patients to better regain
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