RNAi-mediated silencing of Trichinella spiralis serpin-type serine protease inhibitors results in a reduction in larval
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RESEARCH ARTICLE
Open Access
RNAi‑mediated silencing of Trichinella spiralis serpin‑type serine protease inhibitors results in a reduction in larval infectivity Nana Yi, Pengcheng Yu, Lijia Wu, Zhaokun Liu, Jingzhe Guan, Chang Liu, Mingxu Liu and Yixin Lu*
Abstract Trichinella spiralis serpin-type serine protease inhibitors (TsSPIs) are expressed in adult worms (AW), newborn larvae (NBL) and muscle larvae (ML) of T. spiralis, with the ML stage demonstrating the highest expression level. This study aims to determine TsSPI functions in larval viability and invasion of intestinal epithelial cells in vitro, as well as their development, survival, and fecundity in vivo via RNAi. TsSPI-specific siRNAs and dsRNA were transfected into ML by incubation. The silencing effect of TsSPI transcription and expression was determined using qPCR and western blot, respectively. After incubation in 60 ng/μL dsRNA–TsSPI for 3 days, larval TsSPI mRNA and protein expression levels were reduced by 68.7% and 68.4% (P
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