Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion trea
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ORIGINAL ARTICLE
Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment Michael Nemec 1 & Nina Mittinger 2 & Michael Bertl 1 & Emanuela Liu 1 & Erwin Jonke 1 & Oleh Andrukhov 2 Xiaohui Rausch-Fan 1,2
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Received: 5 July 2020 / Accepted: 24 November 2020 # The Author(s) 2020
Abstract Objectives The aim of this study was to investigate changes in saliva concentration of the inflammatory marker MRP-8/14 and the presence of some periodontitis-associated bacteria in patients with mixed dentition treated with a rigid acrylic, bonded maxillary expander. Methods Fifteen patients in mixed dentition treated with a bonded palatal expander were enrolled in this longitudinal study. Saliva samples were taken before the therapy, as well as in 2 weeks and 3, 6, 9, and 12 months after the beginning of the therapy. In each sample, the levels of MRP-8/14 were determined by ELISA and the presence of 11 bacteria was detected by PCR followed by DNA-DNA hybridization. Results Salivary concentration of MRP-8/14 and the amount of Tannerella forsythia, Treponema denticola, and Eikenella corrodens were significantly increased during treatment with bonded maxillary expander. These changes were transient and the maximal levels of MRP-8/14 and periodontitis-associated pathogens were observed 6–9 months after the beginning of the therapy. Conclusion Therapy with bonded maxillary results in higher MRP-8/14 levels and increased prevalence of some periodontitisassociated bacteria, namely T. forsythia, T. denticola, and E. corrodens. The results suggest the detection of salivary MRP-8/14 levels may be a potential tool to reflect the oral health status in children with fixed orthodontic treatment. Clinical relevance Our data suggest that the treatment with bonded maxillary expander might influence the oral health status and should be accompanied by the careful control of the oral health during the therapy. Keywords Saliva . Orthodontic appliances . Oral health . MRP-8/14
Introduction The maintenance of oral health is mainly based on the hostmicrobial homeostasis in the oral cavity. Oral health might be disturbed during the application of different dental devices, particularly fixed orthodontic appliances, which are commonly used to correct malocclusions of various origins. Orthodontic treatment leads to modification and reorientation of periodontal tissues on the one hand, and the parts of
* Oleh Andrukhov [email protected] 1
Division of Orthodontics, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria
2
Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2A, 1090 Vienna, Austria
orthodontic appliances may represent a reservoir for microorganisms and can affect oral microbiota and inflammation levels on the other hand [1, 2]. Additionally, orthodontic treatment with fixed appliances can also hinder oral hygiene due to restricted access to the tooth surface
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