Selective Cytotoxicity of Some Plant Extracts Against Hepatocellular Carcinoma Cells but Not Mesenchymal Stem Cells: A P
Medicinal plants have been used for disease treatment throughout history, especially in Asia. Vietnam is a tropical country which possesses forests with a diversity of plants; among the plants, many have been historically used as alternative therapies for
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Selective Cytotoxicity of Some Plant Extracts Against Hepatocellular Carcinoma Cells but Not Mesenchymal Stem Cells: A Pilot Screening Sinh Truong Nguyen, Nghia Minh Do, Phuc Hong Vo, Mai Thi Thanh Nguyen, Nhan Trung Nguyen, Hai Xuan Nguyen, Kiet Dinh Truong, and Phuc Van Pham Abstract
Introduction Medicinal plants have been used for disease treatment throughout history, especially in Asia. Vietnam is a tropical country which possesses forests with a diversity of plants; among the plants, many have been historically used as alternative therapies for various disease treatments. In this study we aimed to evaluate the selective cytotoxicity of some plant extracts (collected from Vietnamese forests) against hepatocellular carcinoma cells HepG2, compared to adipose tissuederived mesenchymal stem cells (ADSCs).
Methods In this study, we collected nine plants and produced nine extracts from them; these included whole stem of Buchanania lucida, whole stem of Dipterocarpus turbinatus, Hopea recopei, whole stem of Shorea thorelii, bark of Shorea thorelii stem, bark of Dipterocarpus turbinatus stem, whole stem of Dipterocarpus costatus, bark of Dipterocarpus costatus stem, and rhizome of Boesenbergia pandurata. The cytotoxicity of these extracts on hepatocellular carcinoma cells and mesenchymal stem cells were determined based on IC50 values calculated using Alamar Blue assay. Based on these IC50
Sinh Truong Nguyen, Nghia Minh Do, and Phuc Hong Vo Stem Cell Institute, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam
Kiet Dinh Truong Medical Genetic Institute, Ho Chi Minh City, Vietnam e-mail: [email protected]
Cancer Research Laboratory, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam
Phuc Van Pham (*) Stem Cell Institute, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam
Viet Nam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam e-mail: [email protected] Mai Thi Thanh Nguyen, Nhan Trung Nguyen, and Hai Xuan Nguyen Viet Nam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam Chemistry Department, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam e-mail: [email protected]
Cancer Research Laboratory, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam Viet Nam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam Laboratory of Stem Cell Research and Application, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam e-mail: [email protected]; [email protected]; [email protected]
Sinh Truong Nguyen et al.
values, the side effect index (SEI) of extracts was determined. Only the extracts with low SEI were used in further assays to determine the apoptotic status of both hepatocellular carcinoma cells and mesenchymal stem cells via caspase 3/7 induction assay, nuclei disintegration (using Hoechst 33342 staining), and Annexin V staining assay. Results The results showed that B. pandurata extract had strong cytotoxicity toward HepG2 cells with lowest side index on mesenchymal stem cel
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