Selective Fluorometric Analysis of Hg(II) in Industrial Waste Water Samples
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ORIGINAL ARTICLE
Selective Fluorometric Analysis of Hg(II) in Industrial Waste Water Samples Saurabh Kumar Gupta 1 & Kavita Tapadia 1
&
Ashima Sharma 1
Received: 4 August 2020 / Accepted: 24 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The highly selective and sensitive fluorometric method has been developed for trace level determination of Hg(II) is based on photo-induced electron transfer between rhodamine-6G dye and metal complex. Quenching in fluorescence intensity by fluorescence resonance energy transfer (FRET) is due to interaction between metal ion complex and dye. The fluorescence emitted was measured at 510 and 550 nm, for excitation and emission wavelengths respectively. Possible interferences present in water samples, which could affect the analytical response are studied and determined. The calibration graph was dynamically linear from 0.002 to 0.05 mgL−1 of Hg(II) with limit of detection 7 × 10−4 mgL−1 and limit of quantitation 1.9 × 10−3 mgL−1. The SternVolmer constant (KSV) calculated for the quenching of R-6G with Hg (II) was 8.47 Lmg−1 s−1 at optimized reaction conditions. The proposed FRET based fluorometric method was applied successfully in different industrial wastewater samples with satisfactory outcome. Keywords Hg(II) . Rhodamine-6G . Fluorescence . FRET . Quenching . Industrial wastewater samples
Introduction Heavy metal ions are vastly spread in the environment, which play a remarkable role in many biological actions. Among the highly toxic heavy metals, mercury is one of the most studied environmental toxicant having greater impact on ecosystem as well as human wellness. Mercury is believed as the most noxious heavy metal, which is diffused via coal plants, mercury lamps, gold production and many anthropogenic activities. Its exposure leads to major toxicity and long term defect to flora and fauna even in minute level concentration. Toxicity depends upon chemical states of mercury i.e. inorganic mercury is having greater affinity towards sulfhydril moieties of protein. Inorganic mercury causes harmful adverse effects when its concentration reach upto 5000 mgL−1 [1–4]. Hg2+ (mercuric ion) are the most poisonous form of mercury, because of its higher water solubility and stronger affinity towards sulfhydryl groups or thiol (-SH) containing amino
* Kavita Tapadia [email protected] 1
Department of Chemistry, National Institute of Technology, Raipur, CG, India
acids. Its exposure can affect the central nervous system, renal, gastrointestinal, cardiovascular, endocrine and immune system of human being resulting in memory loss, vision problems, paralysis, kidney malfunction, neurological disorder, chromosomal and birth defects etc. In marine environment inorganic mercury get turned into methylmercury which is quite more toxic than the inorganic mercury. Mercury contamination of the environment and its toxic effects has been known for centuries. This allowed the development of convenient and selective analytical strategy for the eff
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