Selective streptavidin bioconjugation on silicon and silicon carbide nanowires for biosensor applications
- PDF / 689,427 Bytes
- 10 Pages / 584.957 x 782.986 pts Page_size
- 112 Downloads / 191 Views
John A. Schreifels Department of Chemistry and Biochemistry, George Mason University, Fairfax, Virginia 22030
Mulpuri V. Rao Department of Electrical and Computer Engineering, George Mason University, Fairfax, Virginia 22030
Albert V. Davydov,b) Vladimir P. Oleshko, Nancy J. Lin, and Kristen L. Steffens Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899
Sergiy Krylyuk Institute for Research in Electronics and Applied Physics, University of Maryland, College Park, Maryland 20742; and Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899
Kris A. Bertness Physical Measurement Laboratory, National Institute of Standards and Technology, Boulder, Colorado 80305
Amy K. Manocchi Sensors and Electronic Devices Directorate, Army Research Lab, Adelphi, Maryland 20783
Yaroslav Koshkac) Department of Electrical and Computer Engineering, Mississippi State University, Mississippi State, Mississippi 39762 (Received 15 April 2012; accepted 6 August 2012)
A functionalization method for the specific and selective immobilization of the streptavidin (SA) protein on semiconductor nanowires (NWs) was developed. Silicon (Si) and silicon carbide (SiC) NWs were functionalized with 3-aminopropyltriethoxysilane (APTES) and subsequently biotinylated for the conjugation of SA. Existence of a thin native oxide shell on both Si and SiC NWs enabled efficient binding of APTES with the successive attachment of biotin and SA as was confirmed with x-ray photoelectron spectroscopy, high-resolution transmission electron microscopy, and atomic force microscopy. Fluorescence microscopy demonstrated nonspecific, electrostatic binding of the SA and the bovine serum albumin (BSA) proteins to APTES-coated NWs. Inhibition of nonspecific BSA binding and enhancement of selective SA binding were achieved on biotinylated NWs. The biofunctionalized NWs have the potential to be used as biosensing platforms for the specific and selective detection of proteins.
I. INTRODUCTION
Over the past decade, there has been an increased interest in the fabrication of chemiresistive-type biosensors that have the ability to selectively detect the binding of label-free biomolecules through a mechanism in which device resistivity changes upon bioconjugation.1–6 Surface Address all correspondence to these authors. a) e-mail: [email protected] b) e-mail: [email protected] c) This author was an editor of this journal during the review and decision stage. For the JMR policy on review and publication of manuscripts authored by editors, please refer to http://www.mrs. org/jmr_policy. DOI: 10.1557/jmr.2012.283 68
J. Mater. Res., Vol. 28, No. 1, Jan 14, 2013
functionalized nanowires (NWs) are ideal active elements for such biosensors due to their high surface-to-volume ratio.5,6 In terms of materials systems, silicon (Si) and silicon carbide (SiC) are attractive semiconductors for inclusion in biosensing devices due to their electronic properties,1,2,6,7 biocompatibility,1–4
Data Loading...
