Separation of microbial oil produced by Mortierella isabellina using polymeric membranes
- PDF / 876,520 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 85 Downloads / 223 Views
RESEARCH PAPER
Separation of microbial oil produced by Mortierella isabellina using polymeric membranes Daniela Sallet1 · Lauren Theisen Fischer1 · Gustavo Ugalde2 · Marcus Vinícius Tres3 · Marcio Antonio Mazutti1 · Raquel Cristine Kuhn1 Received: 15 May 2020 / Accepted: 23 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The objective of this work was to concentrate, through a membrane separation process, the fatty acids from oil/solvent mixture. The oil was obtained by ultrasound-assisted extraction from freeze-dried cells of Mortierella isabellina. The concentration of the fatty acids was investigated using flat-sheet polymer membranes of ultrafiltration and nanofiltration. The effects of temperature and pressure were evaluated by the retention of the fatty acids. Oil retentions between 45.23 and 58.20% to ultrafiltration membrane and 43.50 and 56.00% to nanofiltration membrane were observed. The best condition for the ultrafiltration membrane was 4 bar and 40 °C and for nanofiltration membrane was 12 bar and 50 °C. The oil contains a high concentration of oleic acid and palmitic acid that is a desirable property for the biodiesel production. The results showed the applicability of this technology in the solvent recovery step whereas the oil recovered contains a high concentration of fatty acids. Graphic abstract
Keywords Mortierella isabellina · Ultrafiltration · Nanofiltration · Fatty acids Extended author information available on the last page of the article
13
Vol.:(0123456789)
Introduction Microbial oils, also known as single-cell oils (SCO), can be effectively synthesized and accumulated intracellularly in certain oleaginous microorganisms. Some oleaginous microorganisms can fast accumulate lipids with a fatty acid composition similar to that of vegetable oils and have several advantages over vegetable oils such as a short life cycle and no need for farmland [1, 2]. Mortierella isabellina is an oleaginous fungus capable of accumulating lipids rich in polyunsaturated fatty acids, such as γ linolenic acid, which can be used in dietary supplements and infant nutrition [3]. Besides, the fatty acids produced are myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3) that can be converted into biodiesel through a transesterification reaction [4]. Fatty acids produced by biotechnology are intracellularly and a process of extraction of these lipids is necessary for cell disruption. Therefore, extraction methods such as solvent extraction [5, 6], supercritical fluid extraction [7, 8] and ultrasound-assisted extraction [9, 10] have been applied to obtained these lipids. Sallet et al. [10] and Zhou et al. [9] achieved lipids from M. isabellina by ultrasound-assisted extraction. However, at the end of this extraction a solvent/oil separation process must be performed to separate the lipids. This separation is usually made by solvent evaporation in a fume hood [11], drying [4] or other
Data Loading...
