Proteomic analysis of Mortierella isabellina M6-22 during cold stress
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ORIGINAL PAPER
Proteomic analysis of Mortierella isabellina M6‑22 during cold stress Binbin Hu1 · Minzhou Luo1 · Xiuling Ji1 · Lianbing Lin1 · Yunlin Wei1 · Qi Zhang1
Received: 13 January 2016 / Revised: 29 March 2016 / Accepted: 5 May 2016 © Springer-Verlag Berlin Heidelberg 2016
Abstract We aimed to gain a better understanding of cold adaption in Mortierella isabellina M6-22 by using proteomics approaches. The temperature range and optimal temperature for M6-22 growth were investigated, and composition changes in fatty acids were analyzed. Accompanied with the 2-D gel electrophoresis, MALDI-TOF/TOF– MS analysis was conducted to characterize alterations in protein profiling in M6-22 cultured at 30 °C for 24 h and 15 °C for another 24 h when compared with those cultured at 30 °C for 48 h. Gene Ontology (GO) cluster analysis was finally conducted for successfully identified proteins. M6-22 cells could grow well at temperatures ranging from 15 to 30 °C. As temperature decreased from 30 to 15 °C, LA and GLA significantly increased from 11.63 to 17.85 % and from 9.12 to 13.19 %, respectively, while oleic acid significantly decreased from 47.25 to 36.53 %. Proteomics analyses revealed 111 differentially expressed protein spots, among which 5 unique proteins (A38, A40, A47, A49 and A58), 29 up-regulated proteins and 10 down-regulated proteins were identified by MALDI-TOF/TOF–MS. GO enrichment analysis demonstrated that these proteins mainly involved in glycolytic pathway (A34 and A50), electron transport (A28), ATP production (A35 and B39) and protein modification (A38). A total of 44 differentially
Communicated by Erko Stackebrandt. Binbin Hu and Minzhou Luo should be regarded as co-first authors. * Qi Zhang [email protected] 1
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Kunming 650500, China
expressed proteins have been successfully identified in M. isabellina M6-22 cultured at 15 °C. These proteins may play important roles in cold adaption via regulation of ATP synthesis, activation of cold-adaptive proteins, degradation of needless protein, accumulation of PUFAs, etc. Keywords Mortierella isabellina · Cold stress · Proteomics · Membrane fluidity
Introduction Microbial lipophilic compounds, namely single cell oils (SCO), present potential industrial and financial interests due to their superiority. Mortierella isabellina (M. isabellina) is a kind of microorganism producing SCO, in which lipid accumulation occupies approximately 80 % of cell biomass (Chatzifragkou et al. 2010). M. isabellina is considered as a feedstock for biodiesel production, since its fatty acid composition is similar to those of common plants (Vicente et al. 2009; Economou et al. 2011). What is more, the lipids produced by M. isabellina contain a large amount of high-valued polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA) and arachidonic acid (AA) (Aki et al. 2001; Mamatha et al. 2008), both of which are of nutritional importance and can be used for
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