Silencing LINC00504 inhibits cell proliferation, invasion as well as migration and promotes cell apoptosis in lung cance
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ORIGINAL ARTICLE
Silencing LINC00504 inhibits cell proliferation, invasion as well as migration and promotes cell apoptosis in lung cancer cells via upregulating miR-876-3p Zhen Zhang
Received: 25 April 2020 / Accepted: 18 September 2020 Ó Springer Nature B.V. 2020
Abstract LINC00504 acts as an oncogene and associates with unfavorable prognosis in patients with lung cancer. Silencing LINC00504 may be a promising strategy for treatment of lung cancer and its effects were firstly investigated in lung cancer cells this study. The gene expression level of miR-876-3p as well as LINC00504 were measured via PCR assay. The cell proliferation was investigated through Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Flow cytometry was applied for detection of cell apoptosis. Wound healing and transwell assay were performed for measurement of cell migration and invasion respectively. The apoptosis related protein expressions were measured by western blot. Luciferase report assay was conducted for verification the target gene. LINC00504 was higher expressed in five types of lung cancer cells studied herein when compared with the control normal cells. LINC00504 knockdown exerted inhibitory effects on cell apoptosis, cell migration as well as cell invasion and promoted cell apoptosis. All the effects mentioned above were counteracted by miR-876-3p inhibitor. Silencing LINC00504 possessed anti-proliferation, repression of cell invasion as well as migration and pro-apoptosis effects via targeting up-regulation of
Z. Zhang (&) Cardiovascular thoracic surgery, Fu Yang People’s Hospital, No. 501 Sanqing road, Yingzhou district, Fuyang 236000, Anhui, China e-mail: [email protected]
miR-876-3p in lung cancer cells, proving the new therapeutic targets and highlighting the potential application in future diagnosis and treatment in lung cancer. Keywords LINC00504 miR-876-3p Lung cancer Cell apoptosis Cell invasion and migration
Introduction Lung cancer is one of the major causes of death and the mortality of lung cancer ranks the first among ten leading cancer types, accounting 11.6% of all the cancers (Siegel et al. 2019). In 2019, there were 228,150 new cases of lung cancer, with men more likely to develop the disease than women (Bray et al. 2018; Ferlay et al. 2019; Siegel et al. 2019). Genetic susceptibility as well as smoking was considered to be the major risk factors of lung cancer (Becher et al. 2018; Jassem et al. 2009; Munnia et al. 2017). Smoking cessation, the timely diagnosis and effective treatment are extremely vital for reducing mortality of lung cancer. The early symptoms of lung cancer are not obvious and its underlying pathogenesis is still not fully elucidated. it’s urgent to find new methods for decreasing the incidence and mortality of lung cancer. Long non-coding RNAs (lncRNAs) with over 200 nucleotides exerted aberrant expression in lung cancer
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Cytotechnology
and has regulatory effects via targeting certain type of microRNA (miRNA),
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