Suppression of long non-coding RNA PCAT19 inhibits glioma cell proliferation and invasion, and increases cell apoptosis
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Genes & Genomics (2020) 42:1299–1310 https://doi.org/10.1007/s13258-020-01003-w
RESEARCH ARTICLE
Suppression of long non‑coding RNA PCAT19 inhibits glioma cell proliferation and invasion, and increases cell apoptosis through regulation of MELK targeted by miR‑142‑5p Yu‑hua Xie1 · Jiao Hu2 Received: 26 October 2019 / Accepted: 15 September 2020 / Published online: 26 September 2020 © The Genetics Society of Korea 2020
Abstract Background Glioma has the chief type of primary brain tumors worldwide. The glioma may be controlled by regulators including some lncRNAs, miRNAs, and proteins. Objective Our study aims to discover the underlying mechanism for lncPCAT19/miR-142-5p/MELK axis in glioma progression. Methods The clinical samples were from patients with gliomas in our Hospital. Hematoxylin–eosin staining (H&E) was applied to determine the clinical pathological changes. Real time PCR was performed to measure the levels of lncPCAT19, miR-142-5p, MELK, and expression of other genes. Western blot was conducted to detect the protein level of MELK. RIP assay was performed to analyze the interaction between lncPCAT19 and miR-142-5p, and dual-luciferase reporter assay was used to determine the binding site between lncPCAT19 and miR-142-5p. CCK-8, colony formation assay, flow cytometry, and trans-well assay were carried out to confirm cell proliferation, colony formation, apoptosis, and invasion, respectively. Results LncPCAT19 was increased in cancer tissues. Then, lncPCAT19 could interact with and down-regulate miR-142-5p. Knockdown of lncPCAT19 distinctly inhibited tumor growth in vivo. Interfering lncPCAT19/overexpression of miR-142-5p decreased glioma cell proliferation, colony formation and invasion, and promoted cell apoptosis by down-regulating expression of Cyclin B1, CDK2, N-cadherin, Bcl-2, and by up-regulating expression of Bax and E-cadherin. Moreover, overexpression of lncPCAT19 overturned tumor-suppressing role of miR-142-5p in cells. Additionally, lncPCAT19 and miR-142-5p synergistically regulated expression of MELK. In conclusion, lncPCAT19 enhanced glioma development via increasing MELK by performing as a sponge of miR-142-5p. Conclusions LncPCAT19 promotes glioma progression by sponging miR-142-5p to upregulate MELK levels. Thus, lncPCAT19/miR-142-5p/MELK signaling would be a potential target for glioma treatment. Keywords LncPCAT19 · miR-142-5p · MELK · Xenografts · Glioma
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13258-020-01003-w) contains supplementary material, which is available to authorized users. * Jiao Hu [email protected] 1
Department of Rehabilitation Medicine, Chenzhou No. 1 People’s Hospital, Luo Jia Jin Street, Chenzhou 423000, Hunan, China
Emergency Department, Chenzhou No. 1 People’s Hospital, Luo Jia Jin Street, Chenzhou 423000, Hunan, China
2
Glioma has become the most common type of cerebral cancers or primary brain tumors worldwide (Porter et al. 2010; Jiang et al. 2011). Glioblastoma is the ma
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