Spatiotemporal expression of neurogenic locus notch homolog protein 1 in developing caudal spinal cord of fetuses with a
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ORIGINAL PAPER
Spatiotemporal expression of neurogenic locus notch homolog protein 1 in developing caudal spinal cord of fetuses with anorectal malformations from ETU‑fed rats Zhonghua Yang1 · Huimin Jia1 · Yuzuo Bai1 · Weilin Wang1 Received: 7 January 2020 / Accepted: 25 July 2020 © Springer Nature B.V. 2020
Abstract Complications, such as fecal soiling, incontinence, and constipation, are major health issues for patients with anorectal malformations (ARMs) after surgery. Dysplasia of the caudal spinal cord is an increasingly pivotal area in the field of postoperative complications for patients with ARMs. However, the existing research has not fully defined the mechanism underlying ARMs development. The neurogenic locus notch homolog (Notch) signaling pathway comprises several highly conserved proteins that are involved in spinal cord developmental processes. In the present study, the emerging role of Notch1 in fetal lumbosacral spinal cords was investigated in a rat model of ARMs using ethylene thiourea. Immunohistochemical staining, western blot and quantitative reverse transcription real-time polymerase chain reaction were utilized to analyze spatiotemporal expression of Notch1 on embryonic days (E) 16, E17, E19, and E21. The expression levels of the neuronal marker neurofilament and recombination signal-binding protein-J protein were evaluated for temporal correlations to Notch1 expression. The results implied that Notch1 expression was reduced in lumbosacral spinal cord neurons of ARMs embryos compared to control embryos. These results showed that, in ARMs embryos decreased Notch1 expression is related to the dysplasia of the caudal spinal cord during embryogenesis, indicating that Notch signaling may participate pathogenic embryonic lumbosacral spinal development and may be associated with postoperative complications of ARMs. Keywords Development · Anorectal malformations · Neurons · Lumbosacral spinal cord · Notch1
Introduction Anorectal malformations (ARMs) are a group of rare congenital problems that reportedly occur in one of 4000–5000 births (Herman and Teitelbaum 2012). In the 1980s, advanced work on ARMs was undertaken by Professor Peña, focusing on the anatomy of pelvic muscles and the repair of ARMs with a new technique termed posterior sagittal anorectoplasty (PSARP). This procedure provides definitive reconstruction in ARMs patients and was shown to Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10735-020-09900-w) contains supplementary material, which is available to authorized users. * Weilin Wang [email protected] 1
Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Weilin Wang. 36, Sanhao Street, Heping District, Shenyang 110004, Liaoning, China
decrease postoperative complications. However, despite the long clinical success of PSARP, postoperative complications like fecal soiling, incontinence, and constipation still exist in ARMs patients (Danielson et al. 2017; Zheng et al. 2019). These medical c
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