Stem Cell Heterogeneity Methods and Protocols
Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that st
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Kursad Turksen Editor
Stem Cell Heterogeneity Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes: http://www.springer.com/series/7651
Stem Cell Heterogeneity Methods and Protocols
Edited by
Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
Editor Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6549-6 ISBN 978-1-4939-6550-2 (eBook) DOI 10.1007/978-1-4939-6550-2 Library of Congress Control Number: 2016950593 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface A long-held tenet in the stem cell field was the belief that stem cells—defined by their capacity for self-renewal and lineage developmental capacities—comprised a homogeneous population. Many studies with isolated populations of stem cells were designed and analyzed with this principle in mind. Nevertheless, with time, a variety of types of studies and data suggested that heterogeneous populations or subpopulations of stem cells exist. In this volume, I have pulled together a series of protocols that address different aspects of stem cell heterogeneity. I am very grateful to the contributors for their time and generosity in putting together their concepts and protocols. Once again, I would like to thank Dr. John Walker, Editor in Chief of the Methods in Molecular Biology series, for his support and encouragement. I am grateful to Patrick Marton, Senior Editor of the Methods in Molecular Biology series, for his continuous support throughout the project. A special “thank you” goes to David Casey, Editor of the Methods in Molecular Biology serie
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