Stem Cell Renewal and Cell-Cell Communication Methods and Protocols
This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection pr
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Kursad Turksen Editor
Stem Cell Renewal and Cell-Cell Communication Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
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Stem Cell Renewal and Cell-Cell Communication Methods and Protocols
Edited by
Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
Editor Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2589-6 ISBN 978-1-4939-2590-2 (eBook) DOI 10.1007/978-1-4939-2590-2 Library of Congress Control Number: 2015939235 Springer New York Heidelberg Dordrecht London # Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Two topics—cell–cell interactions and stem cell renewal—are now inexorably linked as we strive to understand the stem cell niche and its function. I have thus selected a number of representative protocols to provide readers with approaches for studying these complimentary aspects of stem cells. As always, I am grateful to the contributors to this volume, for their generosity in sharing their protocols and the numerous details that will help others use these methods to advance their own studies. This volume, as others that I have been involved with, would not have materialized without the strong support and encouragement of the Editor in Chief of the Methods in Molecular Biology series, Dr. John Walker. Similarly, I am grateful to the Executive Editor of Methods in Molecular Biology, Patrick Marton, for being a great cheerleader during the maturation of this volume. Finally, I would like to acknowledge the Editor of Methods
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