Embryonic Stem Cell Protocols
This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages. Research on embryonic stem cells continues to move very quickl
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Kursad Turksen Editor
Embryonic Stem Cell Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Embryonic Stem Cell Protocols Third Edition
Edited by
Kursad Turksen Ottawa Hospital Research Institute, Ottawa, ON, Canada
Editor Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2953-5 ISBN 978-1-4939-2954-2 (eBook) DOI 10.1007/978-1-4939-2954-2 Library of Congress Control Number: 2015951971 Springer New York Heidelberg Dordrecht London # Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Research on embryonic stem cells continues to move very quickly, as reflected in the positive responses that I received for the chapters in this volume, which builds upon a relatively recent previous volume. The kinds of studies continue to expand and diversify, and methodologies are continuously being refined and improved. Amongst areas of intense activity are those related to the very early commitment of stem cells to particular lineages and progression of differentiation to mature cell stages. I am very grateful once again to the contributors who have shared their insights as well as new and improved methods for others already in or just entering the field. I would like to acknowledge Dr. John Walker, Editor in Chief of the Methods in Molecular Biology series, for his continuous support and Patrick Marton, Executive Editor of the series, for encouragement during development of the volume. I am also very grateful to David Casey for being available to answer all of my questions
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