Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis
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ORIGINAL ARTICLE
Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis Yohei Ikezumi 1 & Tomomi Kondoh 1 & Yuji Matsumoto 1 & Naonori Kumagai 1 & Masahiro Kaneko 2 & Hiroya Hasegawa 2 & Takeshi Yamada 2 & Utako Kaneko 2 & David J. Nikolic-Paterson 3 Received: 24 May 2020 / Revised: 17 July 2020 / Accepted: 29 July 2020 # IPNA 2020
Abstract Background M1-type proinflammatory macrophages (MΦ) promote glomerular injury in lupus nephritis (LN). However, whether this phenotype is altered by steroid therapy is unclear. Therefore, we investigated the effect of steroid treatment on MΦ phenotype in LN. Methods Patients with LN (7–18 years old) were divided into 2 groups: those with no treatment (N) before biopsy (n = 17) and those who underwent steroid (S) treatment (3–73 days) before biopsy (n = 15). MΦ number and phenotype were assessed by immunofluorescence. In vitro studies used monocyte-derived MΦ from healthy volunteers. Results Age at biopsy, urine findings, and kidney function (eGFR) were comparable between the two groups. Biopsies in N group had higher levels of active lesions such as endocapillary hypercellularity, necrosis, and cellular crescent formation (p < 0.05). The total CD68+ MΦ infiltrate was comparable between N and S groups. However, N group had more M1 MΦ (CD68+ CD86+ cells) (p < 0.05) and fewer M2 MΦ (CD68+ CD163+ cells) (p < 0.05), giving a 6-fold increase in the M2/M1 ratio in S vs. N groups. Dexamethasone treatment of cultured MΦ induced upregulation of CD163 expression, increased production of anti-inflammatory (IL-10, IL-19) and profibrotic factors (FGF-22, PDGF), and upregulated the scavenger receptor, stabilin-1. Upregulation of stabilin-1 in CD163+ M2 MΦ was confirmed in biopsies from S group. Conclusions Initial steroid treatment induces MΦ phenotypic change from proinflammatory M1 to anti-inflammatory or profibrotic M2 in LN with acute/active lesions. Although steroid treatment is effective for resolution of M1-medated injury, promotion of fibrotic lesions via M2 MΦ is a potential downside of steroid single therapy in LN. Keywords Proinflammatory macrophage . Alternatively activated macrophage . Lupus nephritis . Glucocorticoid . CD86 . CD163 . Children
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00467-020-04734-w) contains supplementary material, which is available to authorized users. * Yohei Ikezumi [email protected] 1
Department of Pediatrics, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake 470-1192, Japan
2
Department of Pediatrics, Niigata University Medical and Dental Hospital, Niigata, Japan
3
Department of Nephrology and Monash University Department of Medicine, Monash Medical Centre, Clayton, Victoria, Australia
Lupus nephritis (LN) is characterized by a wide spectrum of histopathological findings and is a major source of morbidity in systemic lupus erythematosus (SLE) [1]. There is considerable evidence that macropha
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