Structural analysis of cell wall mannan of Candida sojae , a new yeast species isolated from defatted soybean flakes
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ORIGINAL PAPER
Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean Xakes Hiroko Oyamada · Yukiko Ogawa · Nobuyuki Shibata · Yoshio Okawa · Shigeo Suzuki · Hidemitsu Kobayashi
Received: 17 May 2007 / Revised: 26 November 2007 / Accepted: 26 November 2007 / Published online: 15 December 2007 © Springer-Verlag 2007
Abstract We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean Xakes. The results of a slideagglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic Candida species indicated that the cells and the C. sojae mannan were cross-reactive to the speciWc anti-factor sera against Candida albicans serotype A (FAb 6) and Candida guilliermondii (FAb 9). Two-dimensional homonuclear Hartmann–Hahn analysis indicated that the mannan consisted of various linked oligomannosyl side chains containing -1,2-, -1,3-, -1,6- and -1,2-linked mannose residues. However, although the determinants of antigenic factors 6 and 9 could be not found in this mannan, branched side chains, Man1-2Man1-3[Man1-6]Man1-(2Man1-)n2Man and a linear -1,6-linked polymannosyl backbone, which are cross-reacted by FAbs 6 and 9, respectively, were identiWed. The mannan was subjected to acetolysis in order to determine the polymerization length of the -1,2-linked oligomannosyl residue in the side chains. The result of
Communicated by Erko Stackebrandt. H. Oyamada · Y. Ogawa · H. Kobayashi (&) Department of Microbiology, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan e-mail: [email protected] N. Shibata · Y. Okawa Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan S. Suzuki Sendai Research Institute for Mycology, 1-14-34 Tohshogu, Aoba-ku, Sendai, Miyagi 981-0908, Japan
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H-nuclear magnetic resonance analysis of the released oligosaccharides showed that the remarkable regularity in the length of -1,2-linked oligomannosyl side chains, which were previously found in mannans of other Candida species, is not observed in this mannan. Keywords Antigenic factor · Candida sojae · Cell wall mannan · Nuclear magnetic resonance · Polysaccharide antigen Abbreviations ELISA Enzyme-linked immunosorbent assay NMR Nuclear magnetic resonance 2D-HOHAHA Two-dimensional homonuclear Hartmann–Hahn
Introduction An anamorphic yeast species, Candida sojae, was isolated by extraction of water-soluble substances from defatted soybean Xakes. C. sojae was originally described by Nakase et al. (1994). C. sojae resembles the pathogenic yeasts Candida albicans and Candida tropicalis, but diVers from these pathogens by G + C content, DNA–DNA relatedness and electrophoretic enzyme pattern. C. sojae is practically distinguished from C. tropicalis and C. albicans by its inability to ferment maltose and low maximum growt
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