Single Cell Oil Production from Hydrolysates of Inulin by a Newly Isolated Yeast Papiliotrema laurentii AM113 for Biodie
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Single Cell Oil Production from Hydrolysates of Inulin by a Newly Isolated Yeast Papiliotrema laurentii AM113 for Biodiesel Making Guangyuan Wang 1
1
& Lin Liu & Wenxing Liang
2
Received: 8 May 2017 / Accepted: 13 June 2017 # Springer Science+Business Media, LLC 2017
Abstract Microbial oils are among the most attractive alternative feedstocks for biodiesel production. In this study, a newly isolated yeast strain, AM113 of Papiliotrema laurentii, was identified as a potential lipid producer, which could accumulate a large amount of intracellular lipids from hydrolysates of inulin. P. laurentii AM113 was able to produce 54.6% (w/w) of intracellular oil in its cells and 18.2 g/l of dry cell mass in a fed-batch fermentation. The yields of lipid and biomass were 0.14 and 0.25 g per gram of consumed sugar, respectively. The lipid productivity was 0.092 g of oil per hour. Compositions of the fatty acids produced were C14:0 (0.9%), C16:0 (10.8%), C16:1 (9.7%), C18:0 (6.5%), C18:1 (60.3%), and C18:2 (11.8%). Biodiesel obtained from the extracted lipids could be burnt well. This study not only provides a promising candidate for single cell oil production, but will also probably facilitate more efficient biodiesel production. Keywords Papiliotrema laurentii . Single cell oil . Hydrolysates of inulin . Biodiesel
Introduction In recent years, utilization of single cell oils (SCOs) produced by oleaginous microorganisms as source represents a potential interest for biodiesel industries [1, 2]. These microbial oils,
* Guangyuan Wang [email protected] * Wenxing Liang [email protected]
1
College of Life Sciences, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China
2
College of Agronomy and Plant Protection, the Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao 266109, China
Appl Biochem Biotechnol
similar to those of plant oils, have been suggested as a suitable substitute for biodiesel production [3, 4]. Due to global awareness of environmental pollution and energy crisis, more and more researchers have paid close attention to biodiesel production. Compared to the traditional diesel fuel, biodiesel has many advantages, such as renewable, easily degradable, non-toxic, and less emission [5–7]. Although many microorganisms, such as bacteria, filamentous fungi, yeasts, and algae, can accumulate intracellular lipids more than 20% of the dry cell biomass, oleaginous yeasts are the most favorable ones, showing rapid growth rates, without endotoxin, easy to large-scale fermentation, and high oil production [8]. The oleaginous yeasts reported previously belong to various genera, e.g., Candida (ascomycetes), Cryptococcus (basidiomycetes), Lipomyces (Ascomycota), Rhodosporidium (basidiomycetes), Rhodotorula (basidiomycetes), Trichosporon (basidiomycetes), and Yarrowia (Ascomycota) [9–12]. In fact, it is estimated that in the 1600 known yeast species, 50 to 160 of them may be oleaginous
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