Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis , and Study on its Antioxidant A
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Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells Indranil Choudhuri1 · Kalyani Khanra1 · Prasenjit Pariya2 · Gajendra Nath Maity3,4 · Soumitra Mondal5 · Bikas Ranjan Pati6 · Nandan Bhattacharyya1 Received: 31 January 2020 / Accepted: 13 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 105 Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and 1H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1 mg mL−1 of EPS-I as functional as approximately to 500 ± 5.2 µM of ascorbic acid) and Fe2+ metal ion chelation activity (EC50 = 405.6 µg mL−1) and hydroxyl radical scavenging activity (EC50 = 219.5 µg mL−1). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC50 = 267.3 µg mL−1) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02130-z) contains supplementary material, which is available to authorized users. * Nandan Bhattacharyya [email protected] Indranil Choudhuri [email protected] Kalyani Khanra [email protected]
Microorganisms are widely spread in environment, even can survive in extreme environment like high temperature, extremely high or low pH, high salt concentration and heavy metal contaminated areas [1]. Many microorganisms were isolated from industrial effluent, heavy metal enriched soil, 2
Central Inland Fisheries Research Institute, P.O.—Monirampur, Barrackpore, Kolkata, West Bengal 700120, India
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Department of Microbiology, Asutosh College, 92, Shyamaprasad Mukherjee Road, Kolkata, West Bengal 700026, India
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Department of Microbiology, Panskura Banamali College, P.O.—Panskura R.S., Purba Medinipur, West Bengal 721152, India
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Department of Chemistry, Panskura Banamali College, P.O.—Panskura R.S., Purba Medinipur, West Bengal 721152, India
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Department of Microbiology, Vidyasagar University, Paschim Medinipur, West Bengal 721102, India
Prasenjit Pariya [email protected] Gajendra Nath Maity [email protected] Soumitra Mondal [email protected] Bikas Ranjan Pati [email protected] 1
Department of Biotechnology, Panskura Banamali College, P.O.—Panskura R.S., Purba Medinipur, West Bengal 721152, India
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agricultural land, mine, etc., which can tolerate a high concentration of heavy metals [2–6]. Several mechanisms of metal resistance in microorga
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