Studies in Gyromitra I: the Gyromitra gigas species complex
- PDF / 1,610,903 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 40 Downloads / 166 Views
ORIGINAL ARTICLE
Studies in Gyromitra I: the Gyromitra gigas species complex Andrew N. Miller 1 & Angela Yoon 2 & Gro Gulden 3 & Øyvind Stensholt 3 & Nicolas Van Vooren 4 Andrew S. Methven 6
&
Esteri Ohenoja 5 &
Received: 18 August 2020 / Revised: 8 October 2020 / Accepted: 12 October 2020 # German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The Gyromitra gigas species complex includes six morphologically similar taxa, several of which have a long history of segregation, synonymization, and rearrangement among different genera. These taxa occur throughout Asia, Europe, and North America and include G. gigas, G. khanspurensis, G. korfii, G. montana, G. pseudogigas, and G. ticiniana. ITS and LSU sequences from 66 specimens, including type specimens for all six taxa, were included in phylogenetic analyses to establish species boundaries and resolve species relationships. Sequence similarity comparisons were also conducted between the two molecular markers and between the ITS1 and ITS2 regions. Although ITS exhibited sufficient variability to discriminate among species in the G. gigas species complex, LSU displayed very low variability rendering it completely useless as a molecular marker for separating taxa in this group. The ITS1 region was twice as informative as the ITS2 region and can be used as a barcode marker to identify these species. Gyromitra gigas and G. montana occur as a well-supported clade of sister species and can be distinguished based on ascospore morphology. Gyromitra korfii and G. ticiniana also form a highly supported clade and are considered distinct species based on geography. Gyromitra littiniana is confirmed to be synonymous with G. ticiniana based on molecular data. Gyromitra khanspurensis and G. pseudogigas, which also form a highly supported clade, are considered separate species early in the process of speciation that differ significantly in ascomata and ascospore morphology. A key to species based on morphology and geography is provided. Keywords Ascomycota . Fungi . Holotype barcoding . ITS sequences . Systematics
Introduction The genus Gyromitra Fr. contains over 70 taxa commonly referred to as false morels. Members are distributed primarily throughout north temperate and boreal regions of the Northern Section Editor: Roland Kirschner * Andrew N. Miller [email protected] 1
Illinois Natural History Survey, University of Illinois Urbana-Champaign, Champaign, IL, USA
2
Department of Molecular and Cellular Biology, University of Illinois Urbana-Champaign, Champaign, IL, USA
3
Natural History Museum, University of Oslo, Oslo, Norway
4
Tassin-la-Demi-Lune, France
5
Botanical Museum, Biodiversity Unit, University of Oulu, Oulu, Finland
6
Savannah, GA, USA
Hemisphere and form stipitate to sessile ascomata with discoid to cerebriform or saddle-shaped apothecia mostly during the spring. Gyromitra taxa have a long, complex taxonomic history of transfers among various genera (e.g., Discina, Helvella, Maublancomyces, Neogyromitra, a
Data Loading...
