Studies on the Interaction of Water-Soluble Fullerols with BSA and the Effects of Metallic Ions
- PDF / 63,580 Bytes
- 5 Pages / 612 x 792 pts (letter) Page_size
- 24 Downloads / 163 Views
Studies on the Interaction of Water-Soluble Fullerols with BSA and the Effects of Metallic Ions Xu Bingshe1, Liu Xuguang 1, Yan Xiaoqin 1, Qiao Jinli 2 and Jin Weijun3 College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, Shanxi, China, 030024 2 College of Science, Taiyuan University of Technology, Taiyuan, Shanxi, China, 030024 3 Department of Chemistry, Shanxi University, Taiyuan, Shanxi, China, 030006 1
ABSTRACT
The interaction of water-soluble C60 derived fullerols with bovine serum albumin (BSA) in physiological environment was studied in detail by the fluorescence method. Experiments showed that the interaction of fullerols with BAS is mainly in the manner of non-covalent hydrogen bond. Based on the measurements of fluorescence intensity, the apparent binding constant K and the binding site number n were obtained with K=4000 and n=1, and the energy transfer efficiency in the reaction is 0.63. Besides, the effects of metallic ions such as Cu2+, Fe3+ and Cr(VI) on the interaction of fullerols with BSA were investigated. It was found that the effects of the metallic ions are quite different from each other. Low concentrations of Cu2+ can promote the interactions between fullerols and BSA, while high concentrations of Fe3+ or Cr(VI) favorite the interactions between fullerols and BSA.
INTRODUCTION
Fullerene C60 and its derivatives have attracted much interest due to their special structure, and novel chemical and physical functions[1]. However, their water-insoluble property has put much limit on their researches and applications, especially in biochemistry. The success in the synthesis of polyhydroxyl C60 derivative suggests the potential applications in biochemical and pharmaceutically related investigations[2-4]. In previous study, the fluorescence properties of fullerols and their interactions with various metallic ions were studied in detail[5]. Based on the study the fluorescence method was used to investigate the interaction between fullerols and BSA in physiological environment and the effects of various metallic ions, aimed at .understanding the interaction mechanism of fullerols with their biological surroundings.
EXPERIMENTAL
Apparatus and Reagents
All fluorescence measurements were carried out on a Hitachi RF-540 fluorescence W7.4.1
photospectrometer. The wavelengthes of excitation and emission of fullerols were 340/440nm, respectively. Both of the excitation and emission slits were set as 10nm for providing the premium signals. IR spectra were recorded on a Model 1730 infrared photospectrometer, Perking-Elmer Co. and 1HNMR spectra were obtained on a Hitachi R-24B Nuclear Magnetic Resonance photospectrometer for the determination of fullerols structure. C60 (99.9%) was purchased from Tri- Carbon Cluster Materials Co. Ltd., Wuhan University, China. BSA was purchased from Shanghai Lizhu Dongfeng Biotech Co. Ltd., China, with molecular weight of 65000. All chemicals used were analytical reagents. These reagents included the salts giving 3 kinds of ions, such as
Data Loading...
