Sulfated polysaccharide ascophyllan from Padina tetrastromatica enhances healing of burn wounds by ameliorating inflamma
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ORIGINAL ARTICLE
Sulfated polysaccharide ascophyllan from Padina tetrastromatica enhances healing of burn wounds by ameliorating inflammatory responses and oxidative damage Mohsin Sulaiman1,4 · Salem Rashed Alyileili2 · Mahadevan Raghavankutty3,4 · G. Muraleedhara Kurup4 Received: 13 August 2020 / Accepted: 10 October 2020 © Springer Nature B.V. 2020
Abstract Sulfated polysaccharide ascophyllan from marine brown algae has been identified to have burn wound healing properties. Thus, we examined the effects of ascophyllan fraction (AF3) on the inflammatory response and oxidative damage in burn wounds. Full-thickness burn wounds in rats were then treated twice per day with topical AF3 ointment (5%), while control groups were treated with 10% povidone-iodine (positive control) and petroleum jelly-based ointment (negative control). The activity of cyclooxygenase-2 and myeloperoxidase and levels of C-reactive protein, nitric oxide, and proinflammatory cytokines (tumor necrosis factor-α, interleukin-6, and interleukin-1β) were observed to have significantly decreased in peripheral blood mononuclear cells, serum, and wound tissue of the group treated with AF3 ointment on day 8 after wounding. The expression of inducible nitric oxide synthase, endothelial nitric oxide synthase, and vascular endothelial growth factor at the mRNA level was determined to be upregulated in the wound tissue of the AF3 ointment-treated group. After treatment with AF3 ointment, the antioxidant enzyme activity and level of reduced glutathione were upregulated, whereas the content of thiobarbituric acid reactive substances decreased. Treatment of burn wounds using 5% AF3 ointment decreases oxidative damage associated with inflammation deceptively via inhibition of inflammatory enzymes, regulation of proinflammatory cytokines, upregulation of angiogenesis, and activity of antioxidant enzymes. Keywords Cyclooxygenase · Burn wound · Sulfated polysaccharide · Proinflammatory cytokines · Nitric oxide · Antioxidant enzymes
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05914-5) contains supplementary material, which is available to authorized users. * Mohsin Sulaiman [email protected] 1
Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates
2
Department of Integrative Agriculture, College of Food and Agriculture, United Arab Emirates University, P.O. Box 15551, Al Ain, United Arab Emirates
3
Department of Biochemistry and Nutrition, Central Institute of Fisheries Technology, Matsyapuri, P.O. Box 682029, Cochin, Kerala, India
4
Department of Biochemistry, University of Kerala, P.O. Box 695581, Thiruvananthapuram, Kerala, India
Healing of cutaneous wounds is considered as a dynamic physiological process that involves cell restoration. Major events during wound contraction include fibroplasia, migration of fibroblasts and epithelial cells, and angiogenesis [1]. Afte
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