Tandem Mass Tag-based quantitative proteomics analysis of metabolic associated fatty liver disease induced by high fat d
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RESEARCH
Tandem Mass Tag‑based quantitative proteomics analysis of metabolic associated fatty liver disease induced by high fat diet in mice Hu Li1†, Wei Huang1†, Mingjie Wang1, Peizhan Chen2, Li Chen3* and Xinxin Zhang1,2*
Abstract Background: Although metabolic associated fatty liver disease (MAFLD) is the most common chronic liver disease worldwide, the exact molecular mechanism of MAFLD progression remains unknown. In the present study, Tandem Mass Tag-labeled quantitative proteomic technology was used to elucidate the protein expression patterns of liver tissues in the progression of MAFLD, providing new potential therapeutic targets of it. Methods: Five 6-week-old male C57BL/6 mice were fed with high fat diet (HFD) for 22 weeks to establish the MAFLD mouse models. Five C57BL/6 mice of the same age were fed with normal diet (ND) and taken as controls. Mice serum were sampled for biochemical tests, and livers were isolated for histopathological examinations. Six mouse liver samples (three from each group) were performed for proteomic analysis. Differentially expressed proteins were defined using fold change of > 1.5 or
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