The 3-ketoacyl-CoA synthase WFL is involved in lateral organ development and cuticular wax synthesis in Medicago truncat
- PDF / 2,608,698 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 1 Downloads / 138 Views
The 3‑ketoacyl‑CoA synthase WFL is involved in lateral organ development and cuticular wax synthesis in Medicago truncatula Tianquan Yang1,2 · Youhan Li1 · Yu Liu1 · Liangliang He1 · Aizhong Liu3 · Jiangqi Wen4 · Kirankumar S. Mysore5 · Million Tadege5 · Jianghua Chen1 Received: 11 October 2019 / Accepted: 2 October 2020 © Springer Nature B.V. 2020
Abstract Key message A 3-ketoacyl-CoA synthase involved in biosynthesis of very long chain fatty acids and cuticular wax plays a vital role in aerial organ development in M. truncatula. Abstract Cuticular wax is composed of very long chain fatty acids and their derivatives. Defects in cuticular wax often result in organ fusion, but little is known about the role of cuticular wax in compound leaf and flower development in Medicago truncatula. In this study, through an extensive screen of a Tnt1 retrotransposon insertion population in M. truncatula, we identified four mutant lines, named wrinkled flower and leaf (wfl) for their phenotype. The phenotype of the wfl mutants is caused by a Tnt1 insertion in Medtr3g105550, encoding 3-ketoacyl-CoA synthase (KCS), which functions as a rate-limiting enzyme in very long chain fatty acid elongation. Reverse transcription-quantitative PCR showed that WFL was broadly expressed in aerial organs of the wild type, such as leaves, floral organs, and the shoot apical meristem, but was expressed at lower levels in roots. In situ hybridization showed a similar expression pattern, mainly detecting the WFL transcript in epidermal cells of the shoot apical meristem, leaf primordia, and floral organs. The wfl mutant leaves showed sparser epicuticular wax crystals on the surface and increased water permeability compared with wild type. Further analysis showed that in wfl leaves, the percentage of C20:0, C22:0, and C24:0 fatty acids was significantly increased, the amount of cuticular wax was markedly reduced, and wax constituents were altered compared to the wild type. The reduced formation of cuticular wax and wax composition changes on the leaf surface might lead to the developmental defects observed in the wfl mutants. These findings suggest that WFL plays a key role in cuticular wax formation and in the late stage of leaf and flower development in M. truncatula. Keywords KCS · Very long chain fatty acid · Cuticular wax · Organ fusion · Medicago truncatula
Introduction Tianquan Yang and Youhan Li have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01080-1) contains supplementary material, which is available to authorized users. * Jianghua Chen [email protected] 1
2
CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence in Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
Very long chain fatty acids (VLCFAs) with chain l
Data Loading...
