Physiological characterization and gene mapping of a novel cuticular wax-related mutant in barley ( Hordeum vulgare L.)
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ORIGINAL PAPER
Physiological characterization and gene mapping of a novel cuticular wax‑related mutant in barley (Hordeum vulgare L.) Yunxia Fang1 · Xiaoqin Zhang1 · Tao Tong1 · Ziling Zhang1 · Xian Zhang1 · Bin Tian1 · Jun Cui1 · Junjun Zheng1 · Dawei Xue1 Received: 26 July 2020 / Accepted: 2 November 2020 © Springer Nature B.V. 2020
Abstract Cuticular wax is a type of lipid covering the surface of plants, which is directly related to crop stress resistance. Thus, it is important to study wax-related genes and their regulatory mechanism in wax biosynthesis pathway for improving stress resistance. In this study, a wax-deficient barley mutant barley cuticular wax1(bcw1)was identified, and genetic analysis indicated that the trait was controlled by a single recessive nuclear gene. Phenotype observations showed that the tubule-shaped waxy crystals covering the sheath and stem epidermis of mutants disappeared, but there were no significant differences were detected in the leaf epidermis between mutant and wild type. Water loss results confirmed that the cuticular waxes and cutins improved water-holding capacity of plant. By combining the bulk segregant analysis (BSA) and specific locus amplified fragment sequencing (SLAF-seq) strategy, the wax-related gene BCW1 was located on chromosome 2H with a total length of 15.10 Mb. No cuticular wax-related genes have been reported in the 9 regions, indicating that BCW1 is a novel gene that plays roles in cuticular wax biosynthesis and wax crystals formation. Keywords Cuticular waxes · Crystal shapes · Genetic mapping · SLAF-seq · Barley
Introduction Cuticular waxes, primarily composed of very long chain fatty acids(C20-C34) and their derivatives, cover the surface of most organs in the shoots of terrestrial plants (Seo and Park 2011; Bernard and Joubès 2013). Together with the reticular structure cuticle, outside the epidermal cells, cuticular waxes form a hydrophobic barrier for plant selfprotection (Lee and Suh 2015; Yeats and Rose 2015; Xue et al. 2017). As a natural protective layer for plants, cuticular waxes play an important role in the plant response to Yunxia Fang and Xiaoqin Zhang have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10725-020-00680-7) contains supplementary material, which is available to authorized users. * Dawei Xue [email protected] 1
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external biotic and abiotic stresses, such as water loss prevention, high temperature resistance, protection against pathogen invasion and plant-eating insects (Weidenbach et al. 2014; Wang et al. 2015; Xue et al. 2017; Thomas and Puthur 2020). It is important to study the metabolic process of cuticular wax and its related regulatory genes for improving plant stress resistance. Currently, a large number of wax-related genes involved in stress resistance have been isolated and cloned in many plants (Hooker et al. 2
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