The Arabidopsis KH-domain protein FLOWERING LOCUS Y delays flowering by upregulating FLOWERING LOCUS C family members
- PDF / 2,441,300 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 70 Downloads / 203 Views
ORIGINAL ARTICLE
The Arabidopsis KH‑domain protein FLOWERING LOCUS Y delays flowering by upregulating FLOWERING LOCUS C family members Guang‑Yi Dai1 · Ding‑Kang Chen1 · Yun‑Peng Sun1 · Wei‑Yi Liang1 · Yu Liu1 · Li‑Qun Huang1 · Yong‑Kang Li1 · Jia‑Fan He2 · Nan Yao1 Received: 26 May 2020 / Accepted: 7 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message We identified FLY as a previously uncharacterized RNA-binding-family protein that controls flowering time by positively regulating the expression of FLC clade members. The ability of flowering plants to adjust the timing of the floral transition based on endogenous and environmental signals contributes to their adaptive success. In Arabidopsis thaliana, the MADS-domain protein FLOWERING LOCUS C (FLC) and the FLC clade members FLOWERING LOCUS M/MADS AFFECTING FLOWERING1 (FLM/MAF1), MAF2, MAF3, MAF4, and MAF5 form nuclear complexes that repress flowering under noninductive conditions. However, how FLM/ MAF genes are regulated requires further study. Using a genetic strategy, we showed that the previously uncharacterized K-homology (KH) domain protein FLOWERING LOCUS Y (FLY) modulates flowering time. The fly-1 knockout mutant and FLY artificial microRNA knockdown line flowered earlier than the wild type under long- and short-day conditions. The knockout fly-1 allele, a SALK T-DNA insertion mutant, contains an ~ 110-kb genomic deletion induced by T-DNA integration. FLC clade members were downregulated in the fly-1 mutants and FLY artificial microRNA knockdown line, whereas the level of the FLC antisense transcript COOLAIR was similar to that of the wild type. Our results identify FLY as a regulator that affects flowering time through upregulation of FLC clade members. Keywords FLOWERING LOCUS Y · FLC clade members · KH RNA-binding protein · Genome structural variation · Arabidopsis thaliana Abbreviations FLC FLOWERING LOCUS C FLM FLOWERING LOCUS M FLY FLOWERING LOCUS Y HEN4 HUA ENHANCER 4
Communicated by Neal Stewart. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02598-w) contains supplementary material, which is available to authorized users. * Nan Yao [email protected] 1
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People’s Republic of China
School of Agriculture, Sun Yat-Sen University, Guangzhou 510275, People’s Republic of China
2
KH K-homology MAF MADS AFFECTING FLOWERING
Introduction In plants, flowering time is finely tuned by a complicated regulatory network, including the photoperiod, vernalization, autonomous, gibberellic acid (GA), thermosensory, and age-dependent pathways. These pathways respond to changes in day length and light quality, prolonged cold exposure, intrinsic developmental cues, hormonal information, ambient temperature fluctuations, and plant age, respectively (Kim and Sung 2014; Bouche et al. 2017
Data Loading...
