A La-Related Protein LaRP6a Delays Flowering of Arabidopsis thaliana by Upregulating FLC Transcript Levels
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RESEARCH ARTICLE
A La‑Related Protein LaRP6a Delays Flowering of Arabidopsis thaliana by Upregulating FLC Transcript Levels Su Jung Park1 · Hwa Jung Lee1 · Kwanuk Lee1 · Hunseung Kang1 Received: 4 February 2020 / Revised: 1 June 2020 / Accepted: 4 June 2020 © Korean Society of Plant Biologist 2020
Abstract La-related proteins (LaRPs) are RNA-binding proteins (RBPs) that contain a La-type helix-turn-helix (HTH) domain and are found in all eukaryotes, including plants, animals, and yeasts. Although the functions of a few LaRPs have been determined during plant growth and development, the roles of most LaRPs in plants remain unknown. Here, the function of an Arabidopsis thaliana LaRP6a (At5g46250) harboring a La-type HTH domain and an RNA-recognition motif was determined in the growth and development of Arabidopsis. Confocal analysis of subcellular localization of the LaRP6a–GFP fusion protein revealed that LaRP6a protein is localized to both the nucleus and plasma membrane. Overexpression of LaRP6a resulted in delayed flowering and increased plant height and seed yield. The transcript level of the floral repressor FLC was markedly increased in LaRP6a-overexpressing transgenic plants. LaRP6a protein bound to the 3ʹUTR of the FLC transcript and possessed RNA chaperone activity. Collectively, these results suggest that LaRP6a plays a role in the control of flowering time by regulating FLC transcript levels. Keywords Arabidopsis · Flowering · La-related protein · La-type HTH · RNA-binding protein
Introduction Transcriptomic and epitranscriptomic post-transcriptional RNA regulation has been emerging as an important strategy of gene expression regulation (Lee and Kang 2016; Hu et al. 2019; Shen et al. 2019), during which RNA-binding proteins (RBPs) are central cellular factors that regulate RNA metabolism, including RNA processing, intron splicing, RNA editing, mRNA transport, and RNA turnover (Simpson and Filipowicz 1996; Lorković et al. 2000; Qulsum et al. 2019). Typical RBPs contain several well-characterized motifs, domains, or regions, such as an RNA-recognition motif (RRM), zinc-finger motif, K homology domain, glycine-rich Su Jung Park and Hwa Jung Lee contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12374-020-09261-7) contains supplementary material, which is available to authorized users. * Hunseung Kang [email protected] 1
Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, 77 Yongbong‑ro, Buk‑gu, Gwangju 61186, Korea
region, arginine-rich region, and RGG-box region, which contribute to RNA-binding specificity and protein–protein interactions (Lorković and Barta 2002; Lee and Kang 2016). Therefore, the proper arrangement of these motifs, domains, or regions is important for the diverse functions of RBPs in RNA metabolism, which is crucial for plant growth, development, and stress responses (Lorković 2009; Cook et al. 2011; Ambrosone et al. 2012; Jung et al. 2013; Lee and K
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