The chloroplast genome of a rare and an endangered species Salweenia bouffordiana (Leguminosae) in China
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TECHNICAL NOTE
The chloroplast genome of a rare and an endangered species Salweenia bouffordiana (Leguminosae) in China Hafiz Muhammad Wariss1,2,3 · Ting‑Shuang Yi2 · Hong Wang1 · Rong Zhang2,3
Received: 17 July 2017 / Accepted: 9 August 2017 © Springer Science+Business Media B.V. 2017
Abstract Salweenia bouffordiana is an endangered species of Qinghai-Tibetan Plateau (QTP) in China. In this study, its complete plastome was assembled using next-generation sequencing. The complete genome is 153, 730 bp in length, comprises a pair of inverted repeat (IR; 26, 227 bp) regions separated by the large single-copy (LSC; 83, 509 bp) and small single-copy (SSC; 17, 767 bp) regions. It encodes 111 genes, including 77 protein-coding genes (PCGs), 30 tRNAs and 4 rRNAs. The nucleotide composition is asymmetric (31.6% A, 18.1% C, 18.7% G and 31.6% T) with an overall A+T content of 73.2%. Three types of 111 simple sequence repeats (SSRs) were detected in the plastome, including 107 mono- and 4 di-nucleotide repeats. The newly sequenced complete plastome and identified SSRs will help in understanding the plastome evolution and genetic conservation of S. bouffordiana. Keywords Salweenia bouffordiana · Endangered species · Plastome · SSR · Leguminosae
Electronic supplementary material The online version of this article (doi:10.1007/s12686-017-0836-8) contains supplementary material, which is available to authorized users. * Rong Zhang [email protected] 1
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China
2
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China
3
University of Chinese Academy of Sciences, Beijing, China
Salweenia Baker f. is a Tertiary relict genus within the Legumes (Li and Ni 1982; Wang 1989; Wu et al. 2003). It was first described by Baker (1935), confined to dry shrub lands and croaky slopes of the Lancang Jiang, Nu Jiang, and Yalong Jiang valleys of QTP in China (Li and Ni 1982; Ding 1994). Salweenia bouffordiana H. Sun, Z.M. Li & J.P. Yue is an evergreen shrub with beautiful yellow flowers, which occurs within a very narrow range of a fragile habitat in Yalong Jiang, a tributary of the Jinsha Jiang (Yue et al. 2011). Currently, due to the anthropogenic activities, its population is on the verge of rapid decline. The species is critically endangered (CR) (Yue et al. 2011) following the IUCN guidelines (IUCN 2012) and belongs to the rare and endangered plants listed in the category of key protected wild plants in China (http://www.zhb.gov.cn/gkml/ hbb/bgg/201309/t20130912_260061.htm). Considering the importance of the preservation of the precious germplasm, it is necessary to explore available genetic resource for this species. However, chloroplast genome supplies useful molecular markers to reveal genetic diversification, the basis for designing efficient conservation strategies for endangered species (Mukherjee et al. 2016; Twyfor
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