The complete chloroplast genome of wild shaddock, Citrus maxima (Burm.) Merr.
- PDF / 1,630,489 Bytes
- 3 Pages / 595.276 x 790.866 pts Page_size
- 24 Downloads / 217 Views
TECHNICAL NOTE
The complete chloroplast genome of wild shaddock, Citrus maxima (Burm.) Merr. Jin Liu1 · Chao Shi1,2
Received: 9 February 2017 / Accepted: 1 April 2017 © Springer Science+Business Media Dordrecht 2017
Abstract The wild shaddock (Citrus maxima) has been an important species in the development of the fruit breeding program in the genus Citrus, family Rutaceae. However, the majority of its natural resources are subject to deforestation which, accompanied with the lack of efficient conservation efforts in recent decades, has severely shrunk its wild populations and threatened its germplasm resources. In this study, we determined and analyzed the complete chloroplast genome of wild shaddock. It was 160,133 bp in length, containing a pair of 26,845 bp inverted repeat regions (IRs) separated by one large and one small single copy region (SSC and LSC) of 88,043 and 18,400 bp, respectively. Phylogenetic analysis of all sequenced chloroplast genomes in the Rutaceae suggested that shaddock was closely related to three other Citrus species. This complete chloroplast genome will provide valuable information for the development of highly variable DNA markers for future population and conservation studies of wild shaddock. Keywords Citrus maxima · Chloroplast genome · Wild shaddock The genus Citrus boasts the most popular fruits in the Rutaceae family, such as oranges (Citrus sinensis), lemons (C. limon), and mandarins (C. reticulata). The wild shaddock (C. maxima), naturally distributed in South * Chao Shi [email protected] 1
Yunnan Institute of Tropical Crops, Jinghong 666100, China
2
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
China, Thailand, and Indonesia, has provided wild genetic resources for the breeding of many Citrus fruits. However, deforestation and lack of efficient conservation efforts in the last decades have caused severe shrinkage of its wild populations and posed threats to its germplasm resources. Therefore, it is quite urgent to develop highly variable DNA markers that can enable the study of wild shaddock populations and provide conservation genetic recommendations in order to prevent the rapid reduction of its genetic diversity. Chloroplast genome sequences are of great phylogenetic, conservation genetic, and population genetic value due to their relatively conserved structure and comparatively high substitution rates (Ravi et al. 2008). In the genus Citrus, only three chloroplast genomes have been sequenced so far: orange (C. sinensis) (Bausher et al. 2006), C. platymamma (Lee et al. 2015), and lime (C. aurantiifolia) (Su et al. 2014). In this study, we determined the complete chloroplast genome sequence of wild shaddock (GenBank accession number: KY055833), hence adding a new reported chloroplast genome to the genus Citrus. Fresh leaves were collected from the Yunnan Institute of Tropical Crops, Xishuangbanna, China. Total DNA was extracted using the DNeasy Plant Mini Ki
Data Loading...
