The CsGPA1 - CsAQPs module is essential for salt tolerance of cucumber seedlings
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ORIGINAL ARTICLE
The CsGPA1‑CsAQPs module is essential for salt tolerance of cucumber seedlings Yan Yan1 · Mintao Sun1 · Yansu Li1 · Jun Wang1 · Chaoxing He1 · Xianchang Yu1 Received: 18 May 2020 / Accepted: 1 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message CsGPA1 interacts with CsTIP1.1 (a member of CsAQPs) and suppression of CsGPA1 results the reverse expression of CsAQPs in leaves and roots, resulting in declining water content of cucumber seedlings under salt stress. Abstract Salt stress seriously affects cucumber growth and development. Whether the G-protein alpha subunit functions in cucumber during salt stress and its regulation mechanism remains unknown. We interrogated CsGPA1-RNAi lines to identify the role of CsGPA1 during salt stress. Phenotypically, compared with wild type, leaves were severely withered, and root cells showed signs of senescence under salt stress for RNAi lines. Compared with WT, SOD and CAT activity, soluble protein and proline contents all decreased in RNAi lines, while malondialdehyde and relative electrical conductivity increased. Through screening the yeast two-hybrid library and combined with yeast two-hybrid and GST pull-down, the interaction of CsGPA1 with CsTIP1.1 was found the first time in a plant. Then, the expression of aquaporin (AQP) family genes was detected. The expression of CsAQP genes in leaves and roots was primarily up-regulated in WT under salt stress. However, interference by CsGPA1 resulted in enhanced expression of CsAQPs except for CsTIP3.2 in leaves, but reduced expression of some CsAQPs in roots under salt stress. Furthermore, principal component analysis of CsAQP expression profiles and linear regression analysis between CsGPA1 and CsAQPs revealed that CsGPA1 reversely regulated the expression of CsAQPs in leaves and roots under salt stress. Moreover, the water content in leaves and roots of RNAi seedlings significantly decreased compared with WT under salt stress. Overall, CsGPA1 interacts with CsTIP1.1 and suppression of CsGPA1 results in opposite patterns of expression of CsAQPs in leaves and roots, resulting in declining water content of cucumber under salt stress. Keywords Cucumber · Gα subunit · CsAQPs · Salt stress · Rnai
Yan Yan and Mintao Sun contributed equally to this work. The authors responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors are: Xianchang Yu and Chaoxing He. Communicated by Neal Stewart. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02565-5) contains supplementary material, which is available to authorized users.
Introduction The Gα subunit is an integral component of several signal transduction pathways, and in particular, its function has been associated with morphogenesis and abiotic stress (Jangam et al. 2016). Specifically, GPA1 (Guanine nucleotide-binding protein alpha-1 or G-protein alpha-1) has been ch
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