Inoculation of Cucumber ( Cucumis sativus L.) Seedlings with Salt-Tolerant Plant Growth Promoting Bacteria Improves Nutr
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Inoculation of Cucumber (Cucumis sativus L.) Seedlings with Salt‑Tolerant Plant Growth Promoting Bacteria Improves Nutrient Uptake, Plant Attributes and Physiological Profiles Vinodbhai Patel Kartik1 · Hardik Naik Jinal2 · Natarajan Amaresan2 Received: 25 February 2020 / Accepted: 11 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Salinity is one of the major abiotic stresses that cause retarded plant growth and crop production. It has been reported that harmful effects of salinity could be managed by application of salt-tolerant plant growth-promoting (PGP) bacteria. In this study, eight salt-tolerant bacteria were selected from an array of 150 cultures, based on salt-tolerant properties. All the isolates were identified based on 16S rRNA gene sequencing and subjected to different PGP properties. The results showed the production of indole acetic acid (28.83–62.35 µg/ml), ammonia (35.49–67.48 µg/ml), phosphate solubilization (1.45–3.42 µg/ ml), and siderophores. Further, these isolates were evaluated in pot studies on their response in Cucumis sativus plants against 100 mM salinity stress and without salinity stress (normal water) along with uninoculated controls. The results revealed that bacterized seeds had enhanced plant growth parameters and there was a corresponding increase in biomass in both conditions compared to control plants. The maximum enhancement in root and shoot length under normal condition was 54.6% (SBR-4) and 69.3% (SBS-19), whereas under saline conditions it was 42.3% (SBR-5) and 100.4% (SBS-18), respectively. Further, the inoculated seedlings maximum enhanced chlorophyll a (SBS-3; 50.6% and SBR-4; 45.4%) and chlorophyll b (SBR-1; 83.6% and SBS-3; 209.6%), proline (SBS-18; 62.0% and SBR-1; 32.8%), total phenol (SBR-1; 48.2% and SBR-5; 67.8%), ascorbic acid (SBS-2; 118.6% and SBR-1; 22.4%), and shoot and root N and P contents (twofold to threefold) under both normal and saline conditions. Among the eight isolates, isolates SBS-19, SRB-5, SBS-18, and SBR-4 improved plant growth and other parameters under both conditions in C. sativus plants. Therefore, it is suggested that these salt-tolerant PGP bacteria may be used for cultivation of C. sativus in salinized agricultural lands. Keywords Antioxidant · Cucumber · Nitrogen uptake · Phosphorus uptake · Plant growth promotion · Salinity · Siderophore
Introduction In the recent times, plant growth and yield severely affected due to various abiotic environmental stresses such as soil salinity, extreme temperatures, drought, high winds, and floods (Pandey et al. 2017). Among the abiotic stresses, soil salinity is considered as a highly destructive environmental
* Natarajan Amaresan [email protected] 1
Department of Biosciences, Veer Narmad South Gujarat University, Surat, Gujarat, India
C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus, Bardoli, Surat, Gujarat 394 350, India
2
stress, which leads to major reductions in crop yields, cultivated land area, and qu
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