The dsRNA mycovirus ChNRV1 causes mild hypervirulence in the fungal phytopathogen Colletotrichum higginsianum
- PDF / 1,222,181 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 21 Downloads / 155 Views
ORIGINAL PAPER
The dsRNA mycovirus ChNRV1 causes mild hypervirulence in the fungal phytopathogen Colletotrichum higginsianum Marta Olivé1 · Sonia Campo1 Received: 9 March 2020 / Revised: 21 August 2020 / Accepted: 2 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The genus Colletotrichum comprises a large number of filamentous fungi responsible for anthracnose diseases in many tropical and subtropical fruits and vegetables. In particular, Colletotrichum higginsianum infects Brassicaceae species, including Arabidopsis. The C. higginsianum strain IMI349063A is naturally infected with a dsRNA virus, named Colletorichum higginsianum non-segmented virus (ChNRV1). Here, we investigated the biological effect of ChNRV1 in C. higginsianum by comparing strains with and without the virus. ChNRV1 does not have an effect on C. higginsianum growth under salt and cell-wall stress conditions. However, thermal stress reduced C. higginsianum growth rate, this effect being more evident in the wild-type C. higginsianum strain containing the virus. Although ChNRV1 had no effect in conidiation, conidia were narrower when the virus is present. More importantly, ChNRV1 causes a mild increase in C. higginsianum virulence (hypervirulence) when infecting Arabidopsis plants. These findings indicated that, whereas the ChNRV1 mycovirus does not impair growth and conidiation of C. higginsianum, it confers hypervirulence to the fungal host. These findings will help in future research on the effect of mycoviral infection on pathogenic fungi in plant species of agronomical relevance. Keywords Colletotrichum · Mycovirus · Virulence · Arabidopsis · Conidiation Abbreviations ChNRV1 Colletotrichum higginsianum non-segmented dsRNA virus 1 dpi Days post-infection ds Double stranded ss Single stranded SAR Systemic acquired resistance
Communicated by Erko stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02030-7) contains supplementary material, which is available to authorized users. * Sonia Campo [email protected] Marta Olivé [email protected] 1
Centre for Research in Agricultural Genomics (CRAG) CSIC‑IRTA‑UAB‑UB, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain
Introduction Fungal viruses, or mycoviruses, are widespread in all major groups of phytopathogenic fungi. Most mycoviruses have double-stranded RNA (dsRNA) genomes, but single-stranded (ssRNA) and DNA genomes have also been described (Yu et al. 2010; Ghabrial et al. 2015; Son et al. 2015). They replicate within fungal cells and are generally transmitted via spores (vertical transmission) or anastomosis (horizontal transmission). Thus, their entire life cycle occurs exclusively within their host cell. Evidence also support that some mycoviruses can be transmitted between vegetative incompatible strains, such as those infecting the plant fungal pathogen Sclerotinia sclerotium (Hamid et al. 2018). Also, extracell
Data Loading...
